Olayemi M Adegbolagun1, Benjamin O Emikpe2, Ibukun Oluwa O Woranola1, Yetunde Ogunremi3. 1. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Ibadan. 2. Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ibadan, Nigeria. 3. Department of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmacy, University of Ibadan.
Abstract
BACKGROUND: Resistance to most antimalarial drugs has encouraged the use of herbal preparations along with prescribed orthodox drugs. OBJECTIVE: this study investigated effect of co-administration of aqueous extract of T. occidentalis leaves, commonly used as antimalarial and haematinic agent in Nigeria, and artesunate using P. berghei animal model. METHODS: In vivo curative antiplasmodial effect of T. occidentalis (200mg/kg) alone and in combination with artesunate (2mg/kg) were evaluated using albino mice infected with 10(6) parasitized erythrocytes of P. berghei intraperitoneally. The haematological parameters: haemoglobin level, red blood cells and white blood cells and packed cell volume were monitored using standard methods. RESULTS: Aqueous extract of T. occidentalis, artesunate and the combination gave 72.17±4.07%, 70.43± 4.27% and 85.43±3.65% reduction in parasitaemia after 48hours respectively. A significant enhancement of the PCV was obtained with the co-administration of artesunate and aqueous extract (p < 0.01). Similar trends were also observed with heamatological parameters at 72 hours of administration. CONCLUSION: This study revealed a synergistic effect of the co-administration on parasite clearance rate of P. berghei infection in mice, with a significant enhancement of haematological parameters within 48 hours of administration. This indicates a rapid rate of recovery from plasmodial infections with the co-administration.
BACKGROUND: Resistance to most antimalarial drugs has encouraged the use of herbal preparations along with prescribed orthodox drugs. OBJECTIVE: this study investigated effect of co-administration of aqueous extract of T. occidentalis leaves, commonly used as antimalarial and haematinic agent in Nigeria, and artesunate using P. berghei animal model. METHODS: In vivo curative antiplasmodial effect of T. occidentalis (200mg/kg) alone and in combination with artesunate (2mg/kg) were evaluated using albino mice infected with 10(6) parasitized erythrocytes of P. berghei intraperitoneally. The haematological parameters: haemoglobin level, red blood cells and white blood cells and packed cell volume were monitored using standard methods. RESULTS: Aqueous extract of T. occidentalis, artesunate and the combination gave 72.17±4.07%, 70.43± 4.27% and 85.43±3.65% reduction in parasitaemia after 48hours respectively. A significant enhancement of the PCV was obtained with the co-administration of artesunate and aqueous extract (p < 0.01). Similar trends were also observed with heamatological parameters at 72 hours of administration. CONCLUSION: This study revealed a synergistic effect of the co-administration on parasite clearance rate of P. berghei infection in mice, with a significant enhancement of haematological parameters within 48 hours of administration. This indicates a rapid rate of recovery from plasmodial infections with the co-administration.
Authors: Mohamed A Dkhil; Saleh Al-Quraishy; Esam M Al-Shaebi; Rewaida Abdel-Gaber; Felwa Abdullah Thagfan; Mahmood A A Qasem Journal: Saudi J Biol Sci Date: 2020-12-19 Impact factor: 4.219