Can Temiz1, Serdar Kalemci2, Serap Cilaker Micili3, Isil Tekmen3, Gulserap Yildiz4, Tolgahan Acar5, Sibel Ayik6, Saliha Aksun7, Osman Yilmaz8, Atila Akkoclu1. 1. Department of Chest Diseases, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey. 2. Department of Chest Diseases, Faculty of Medicine, Mugla University, Mugla, Turkey. 3. Department of Histology, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey. 4. Department of Biochemistry, Health Sciences Institute of Adnan Menderes University, Aydin, Turkey. 5. Department of Anatomy, Faculty of Medicine, Sifa University, Izmir, Turkey. 6. Department of Chest Diseases, Faculty of Medicine, Katip Celebi University, Izmir, Turkey. 7. Department of Biochemistry, Faculty of Medicine, Katip Celebi University, Izmir, Turkey. 8. Multidisciplinary Laboratory, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
Abstract
OBJECTIVE: To determine the effects of methyl palmitate on murine model of chronic asthma. METHODS: The experimental study was conducted in the animal laboratory of DokuzEylul University, Turkey, from October to December, 2012, and comprised BALB/c mice whowere divided into four equal groups: three experimental and one control group. All groups except the control group were sensitised and challenged with ovalbumin. Mice with experimentally-induced asthma in Group I received saline; Group II dexamethasone 1mg/kg; Group III methyl palmitate300mg/kg intraperitoneally three times per week in the last four weeks of the study period. Animals were sacrificed 24h after the last administration of study drugs. Histological findings of airways were evaluated by light microscopic examination. Blood samples from vena cava inferior were taken for measurement of interleukin-5 levels. SPSS 15 was used for statistical analysis. RESULTS: The 28 female mice in the study were divided into 4 groups of 7(25%) each. The age range of the animals was 6-8weeks, and the weight range was 18-20g. All histological parameters and interleukin-5 levels of asthma in the Group III were significantly ameliorated compared to the Group I (p<0.05). All histological parameters and interleukin-5 levels were similar between Group III and Group II. CONCLUSIONS: Methyl palmitate exhibited anti-inflammatory effects by resolving the histological changes and reducing the interleukin-5 levels in murine model of chronic asthma.
OBJECTIVE: To determine the effects of methyl palmitate on murine model of chronic asthma. METHODS: The experimental study was conducted in the animal laboratory of DokuzEylul University, Turkey, from October to December, 2012, and comprised BALB/c mice whowere divided into four equal groups: three experimental and one control group. All groups except the control group were sensitised and challenged with ovalbumin. Mice with experimentally-induced asthma in Group I received saline; Group II dexamethasone 1mg/kg; Group III methyl palmitate300mg/kg intraperitoneally three times per week in the last four weeks of the study period. Animals were sacrificed 24h after the last administration of study drugs. Histological findings of airways were evaluated by light microscopic examination. Blood samples from vena cava inferior were taken for measurement of interleukin-5 levels. SPSS 15 was used for statistical analysis. RESULTS: The 28 female mice in the study were divided into 4 groups of 7(25%) each. The age range of the animals was 6-8weeks, and the weight range was 18-20g. All histological parameters and interleukin-5 levels of asthma in the Group III were significantly ameliorated compared to the Group I (p<0.05). All histological parameters and interleukin-5 levels were similar between Group III and Group II. CONCLUSIONS:Methyl palmitate exhibited anti-inflammatory effects by resolving the histological changes and reducing the interleukin-5 levels in murine model of chronic asthma.