Literature DB >> 26060070

Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates.

Benjamin J Tillotson1, Jason M Lajoie, Eric V Shusta.   

Abstract

It is often desired to identify or engineer antibodies that target membrane proteins (MPs). However, due to their inherent insolubility in aqueous solutions, MPs are often incompatible with in vitro antibody discovery and optimization platforms. Recently, we adapted yeast display technology to accommodate detergent-solubilized cell lysates as sources of MP antigens. The following protocol details the incorporation of cell lysates into a kinetic screen designed to obtain antibodies with improved affinity via slowed dissociation from an MP antigen.

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Year:  2015        PMID： 26060070      PMCID： PMC5076467          DOI： 10.1007/978-1-4939-2748-7_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  27 in total

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Journal:  Biochim Biophys Acta       Date:  2000-11-23

2.  Resistance of cell membranes to different detergents.

Authors:  Sebastian Schuck; Masanori Honsho; Kim Ekroos; Andrej Shevchenko; Kai Simons
Journal:  Proc Natl Acad Sci U S A       Date:  2003-04-29       Impact factor: 11.205

3.  Drug-target network.

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Journal:  Nat Biotechnol       Date:  2007-10       Impact factor: 54.908

Review 4.  Cells and cell lysates: a direct approach for engineering antibodies against membrane proteins using yeast surface display.

Authors:  Benjamin J Tillotson; Yong Ku Cho; Eric V Shusta
Journal:  Methods       Date:  2012-03-23       Impact factor: 3.608

5.  Directed evolution for improved secretion of cancer-testis antigen NY-ESO-1 from yeast.

Authors:  Andrea Piatesi; Shanshan W Howland; James A Rakestraw; Christoph Renner; Neil Robson; Jonathan Cebon; Eugene Maraskovsky; Gerd Ritter; Lloyd Old; K Dane Wittrup
Journal:  Protein Expr Purif       Date:  2006-03-02       Impact factor: 1.650

6.  Yeast surface display for directed evolution of protein expression, affinity, and stability.

Authors:  E T Boder; K D Wittrup
Journal:  Methods Enzymol       Date:  2000       Impact factor: 1.600

7.  Recycling kinetics and transcytosis of transferrin in primary cultures of bovine brain microvessel endothelial cells.

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Journal:  J Cell Physiol       Date:  1991-10       Impact factor: 6.384

8.  From XenoMouse technology to panitumumab, the first fully human antibody product from transgenic mice.

Authors:  Aya Jakobovits; Rafael G Amado; Xiaodong Yang; Lorin Roskos; Gisela Schwab
Journal:  Nat Biotechnol       Date:  2007-10       Impact factor: 54.908

9.  An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues.

Authors:  M Zaccolo; D M Williams; D M Brown; E Gherardi
Journal:  J Mol Biol       Date:  1996-02-02       Impact factor: 5.469

10.  Beyond natural antibodies: the power of in vitro display technologies.

Authors:  Andrew R M Bradbury; Sachdev Sidhu; Stefan Dübel; John McCafferty
Journal:  Nat Biotechnol       Date:  2011-03       Impact factor: 54.908
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  7 in total

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3.  Magnetic Bead-Immobilized Mammalian Cells Are Effective Targets to Enrich Ligand-Displaying Yeast.

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Authors:  Benjamin J Umlauf; Kalie A Mix; Vanessa A Grosskopf; Ronald T Raines; Eric V Shusta
Journal:  Bioconjug Chem       Date:  2018-05-03       Impact factor: 4.774

5.  Extended yeast surface display linkers enhance the enrichment of ligands in direct mammalian cell selections.

Authors:  Patrick S Lown; Jessy J Cai; Seth C Ritter; Jacob J Otolski; Ryan Wong; Benjamin J Hackel
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6.  The Antitumor Activity of TCR-Mimic Antibody-Drug Conjugates (TCRm-ADCs) Targeting the Intracellular Wilms Tumor 1 (WT1) Oncoprotein.

Authors:  Ying Shen; Yi-Ming Li; Jing-Jing Zhou; Zhan Zhou; Ying-Chun Xu; Wen-Bin Zhao; Shu-Qing Chen
Journal:  Int J Mol Sci       Date:  2019-08-12       Impact factor: 5.923

7.  Correlating single-molecule rupture mechanics with cell population adhesion by yeast display.

Authors:  Mariana Sá Santos; Haipei Liu; Valentin Schittny; Rosario Vanella; Michael A Nash
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  7 in total

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