31P NMR studies of excised gray and white calf brain.

1. 31P NMR examination of isolated calf gray and white matter reveals that white matter contains higher levels of the phosphodiester glycerolphosphoryl choline (GPC) than gray. 2. It is suggested that GPC may play a role in maintaining the level of phospholipids present by inhibition of phospholipases. 3. The spectra also reveal a skewed peak whose maximum is at -11 ppm which is inferred to arise from myelin-like structures. 4. The results show that phosphorus spectra from the brain must be carefully considered whether they arise from the same type tissue or represent a mixed sample since variation in results may represent anatomy as well as physiology.