Kais Mnafgui1,2, Ines Khlif3, Raouf Hajji2, Fatma Derbali2, Ftouma Kraiem4, Hedi Ellefi5, Thomas Michel6,7, Maria Halabalaki7, Alexios Leandros Skaltsounis7, Abdelfattah Elfeki1, Neji Gharsallah8, Noureddine Allouche3. 1. a Faculty of Sciences of Sfax , Laboratory of Animal Physiology, University of Sfax , Sfax , Tunisia . 2. b Department of Internal Medicine , Hospital of Sidi Bouzid , Sidi Bouzid , Tunisia . 3. c Faculty of Sciences of Sfax , Laboratory of Plant Biotechnology , Sfax , Tunisia . 4. d Faculty of Medicine of Monastir , Monastir , Tunisia . 5. e Department of Cardiology , Centre Hospitalier Intercommunal Robert Ballanger , Aulnay-Sous-Bois , France . 6. f ICN, Université Nice Sophia Antipolis , Cedex , France . 7. g Laboratory of Pharmacognosy and Natural Products Chemistry , School of Pharmacy, University of Athens , Athens , Greece , and. 8. h Faculty of Sciences of Sfax , Laboratory of Chemistry of Natural Products , Sfax , Tunisia.
Abstract
OBJECTIVE: Myocardial infarction remains the major cause of global death due to cardiovascular diseases. This study aimed to assess the protective role of oleuropein in attenuating the cardiac remodeling in isoproterenol-induced myocardial infarction in rats. METHODS AND RESULTS: Male Wistar rats were randomly divided into four groups, control, isoproterenol (Isop) and pretreated animals with oleuropein at two different doses (20 and 40 mg/kg) orally for 7 days and intoxicated with isoproterenol (Isop+Oleu20) and (Isop+Oleu40) groups. The subcutaneous injection of isoproterenol (100 mg/kg body weight) to untreated rats for two consecutive days showed significant increases in ST-segment elevation, heart weight index and alteration in the ECG pattern and hemodynamic function. Else, serum levels of cardiac troponin-T, creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) underwent a notable rise in serum of Isop group by (345, 82, 73 and 106%, respectively) as compared to normal rats. Isoproterenol-induced myocardial injury was evidenced by alteration in serum lipids profile and increased activities of pancreatic lipase by 94% and angiotensin-converting enzyme (ACE) by 78% which reflects the occurrence of cardiac remodeling process. The histopathological findings of the infarcted group showed myocardium necrosis and cells inflammatory infiltration. However, the treatment with oleuropein gave a good protection of the myocardium by decreasing cardiac injury markers specially troponin-T, restoring hemodynamic parameters and attenuating cardiac remodeling process through inhibition of ACE activity. CONCLUSION: Oleuropein offers high preventive effects from cardiac remodeling process in rats with acute myocardial infarction.
OBJECTIVE:Myocardial infarction remains the major cause of global death due to cardiovascular diseases. This study aimed to assess the protective role of oleuropein in attenuating the cardiac remodeling in isoproterenol-induced myocardial infarction in rats. METHODS AND RESULTS: Male Wistar rats were randomly divided into four groups, control, isoproterenol (Isop) and pretreated animals with oleuropein at two different doses (20 and 40 mg/kg) orally for 7 days and intoxicated with isoproterenol (Isop+Oleu20) and (Isop+Oleu40) groups. The subcutaneous injection of isoproterenol (100 mg/kg body weight) to untreated rats for two consecutive days showed significant increases in ST-segment elevation, heart weight index and alteration in the ECG pattern and hemodynamic function. Else, serum levels of cardiac troponin-T, creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) underwent a notable rise in serum of Isop group by (345, 82, 73 and 106%, respectively) as compared to normal rats. Isoproterenol-induced myocardial injury was evidenced by alteration in serum lipids profile and increased activities of pancreatic lipase by 94% and angiotensin-converting enzyme (ACE) by 78% which reflects the occurrence of cardiac remodeling process. The histopathological findings of the infarcted group showed myocardium necrosis and cells inflammatory infiltration. However, the treatment with oleuropein gave a good protection of the myocardium by decreasing cardiac injury markers specially troponin-T, restoring hemodynamic parameters and attenuating cardiac remodeling process through inhibition of ACE activity. CONCLUSION: Oleuropein offers high preventive effects from cardiac remodeling process in rats with acute myocardial infarction.
Authors: Sameer N Goyal; Umesh B Mahajan; Govind Chandrayan; Vivek S Kumawat; Sarika Kamble; Pradip Patil; Yogeeta O Agrawal; Chandragouda R Patil; Shreesh Ojha Journal: Am J Transl Res Date: 2016-01-15 Impact factor: 4.060
Authors: Andrés Jiménez-Sánchez; Antonio Jesús Martínez-Ortega; Pablo Jesús Remón-Ruiz; Ana Piñar-Gutiérrez; José Luis Pereira-Cunill; Pedro Pablo García-Luna Journal: Nutrients Date: 2022-03-31 Impact factor: 5.717