| Literature DB >> 26056849 |
Aurélien Laguerre1, Kyle Hukezalie2, Pascale Winckler3, Fares Katranji1, Gaëtan Chanteloup1, Marc Pirrotta1, Jean-Marie Perrier-Cornet3, Judy M Y Wong2, David Monchaud1.
Abstract
Visualization of DNA and RNA quadruplex formation in human cells was demonstrated recently with different quadruplex-specific antibodies. Despite the significant interest in these immunodetection approaches, dynamic detection of quadruplex in live cells remains elusive. Here, we report on NaphthoTASQ (N-TASQ), a next-generation quadruplex ligand that acts as a multiphoton turn-on fluorescent probe. Single-step incubation of human and mouse cells with N-TASQ enables the direct detection of RNA-quadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a unique, unbiased visualization of quadruplexes in live cells.Entities:
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Year: 2015 PMID： 26056849 DOI： 10.1021/jacs.5b03413
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419