Literature DB >> 26055071

A perfusion-capable microfluidic bioreactor for assessing microbial heterologous protein production.

Nicholas J Mozdzierz1, Kerry R Love, Kevin S Lee, Harry L T Lee, Kartik A Shah, Rajeev J Ram, J Christopher Love.   

Abstract

We present an integrated microfluidic bioreactor for fully continuous perfusion cultivation of suspended microbial cell cultures. This system allowed continuous and stable heterologous protein expression by sustaining the cultivation of Pichia pastoris over 11 days. This technical capability also allowed testing the impact of perfusion conditions on protein expression. This advance should enable small-scale models for process optimization in continuous biomanufacturing.

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Year:  2015        PMID： 26055071      PMCID： PMC4627644          DOI： 10.1039/c5lc00443h

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  25 in total

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Authors:  Veena Warikoo; Rahul Godawat; Kevin Brower; Sujit Jain; Daniel Cummings; Elizabeth Simons; Timothy Johnson; Jason Walther; Marcella Yu; Benjamin Wright; Jean McLarty; Kenneth P Karey; Chris Hwang; Weichang Zhou; Frank Riske; Konstantin Konstantinov
Journal:  Biotechnol Bioeng       Date:  2012-08-06       Impact factor: 4.530

2.  In situ measurement of bioluminescence and fluorescence in an integrated microbioreactor.

Authors:  Andrea Zanzotto; Paolo Boccazzi; Nathalie Gorret; Tina K Van Dyk; Anthony J Sinskey; Klavs F Jensen
Journal:  Biotechnol Bioeng       Date:  2006-01-05       Impact factor: 4.530

3.  A microfluidic chemostat for experiments with bacterial and yeast cells.

Authors:  Alex Groisman; Caroline Lobo; HoJung Cho; J Kyle Campbell; Yann S Dufour; Ann M Stevens; Andre Levchenko
Journal:  Nat Methods       Date:  2005-09       Impact factor: 28.547

4.  Automated disposable small scale reactor for high throughput bioprocess development: a proof of concept study.

Authors:  Rachel Bareither; Neil Bargh; Robert Oakeshott; Kathryn Watts; David Pollard
Journal:  Biotechnol Bioeng       Date:  2013-07-01       Impact factor: 4.530

5.  Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter.

Authors:  H R Waterham; M E Digan; P J Koutz; S V Lair; J M Cregg
Journal:  Gene       Date:  1997-02-20       Impact factor: 3.688

6.  Microchemostat-microbial continuous culture in a polymer-based, instrumented microbioreactor.

Authors:  Zhiyu Zhang; Paolo Boccazzi; Hyun-Goo Choi; Gerardo Perozziello; Anthony J Sinskey; Klavs F Jensen
Journal:  Lab Chip       Date:  2006-04-20       Impact factor: 6.799

Review 7.  Heterologous protein expression in the methylotrophic yeast Pichia pastoris.

Authors:  J L Cereghino; J M Cregg
Journal:  FEMS Microbiol Rev       Date:  2000-01       Impact factor: 16.408

8.  The "push-to-low" approach for optimization of high-density perfusion cultures of animal cells.

Authors:  Konstantin Konstantinov; Chetan Goudar; Maria Ng; Renato Meneses; John Thrift; Sandy Chuppa; Cary Matanguihan; Jim Michaels; David Naveh
Journal:  Adv Biochem Eng Biotechnol       Date:  2006       Impact factor: 2.635

9.  Plastic-PDMS bonding for high pressure hydrolytically stable active microfluidics.

Authors:  Kevin S Lee; Rajeev J Ram
Journal:  Lab Chip       Date:  2009-03-13       Impact factor: 6.799

10.  Microbial factories for recombinant pharmaceuticals.

Authors:  Neus Ferrer-Miralles; Joan Domingo-Espín; José Luis Corchero; Esther Vázquez; Antonio Villaverde
Journal:  Microb Cell Fact       Date:  2009-03-24       Impact factor: 5.328
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  3 in total

Review 1.  Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development.

Authors:  Nagesh K Tripathi; Ambuj Shrivastava
Journal:  Front Bioeng Biotechnol       Date:  2019-12-20

2.  Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care.

Authors:  Pablo Perez-Pinera; Ningren Han; Sara Cleto; Jicong Cao; Oliver Purcell; Kartik A Shah; Kevin Lee; Rajeev Ram; Timothy K Lu
Journal:  Nat Commun       Date:  2016-07-29       Impact factor: 14.919

3.  Enhancing the functionality of a microscale bioreactor system as an industrial process development tool for mammalian perfusion culture.

Authors:  David J Sewell; Richard Turner; Ray Field; William Holmes; Rahul Pradhan; Christopher Spencer; Stephen G Oliver; Nigel Kh Slater; Duygu Dikicioglu
Journal:  Biotechnol Bioeng       Date:  2019-02-20       Impact factor: 4.530
  3 in total

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