Dorothée Poidatz1, Esther Dos Santos2, Fabien Duval3, Hadia Moindjie3, Valérie Serazin2, François Vialard4, Philippe De Mazancourt5, Marie-Noëlle Dieudonné3. 1. Unité de Pathologie Cellulaire et Génétique - Equipe d'Accueil 2493, Université de Versailles-St. Quentin, Unité de Formation et de Recherche des Sciences de la Santé, Montigny le Bretonneux, France. Electronic address: dorothee.poidatz@neuf.fr. 2. Unité de Pathologie Cellulaire et Génétique - Equipe d'Accueil 2493, Université de Versailles-St. Quentin, Unité de Formation et de Recherche des Sciences de la Santé, Montigny le Bretonneux, France; Service de Biologie Médicale, Centre Hospitalier Intercommunal (CHI) de Poissy-St-Germain, Poissy, France. 3. Unité de Pathologie Cellulaire et Génétique - Equipe d'Accueil 2493, Université de Versailles-St. Quentin, Unité de Formation et de Recherche des Sciences de la Santé, Montigny le Bretonneux, France. 4. Unité de Pathologie Cellulaire et Génétique - Equipe d'Accueil 2493, Université de Versailles-St. Quentin, Unité de Formation et de Recherche des Sciences de la Santé, Montigny le Bretonneux, France; Département de Biologie de la Reproduction, Cytogénétique, Gynécologie et Obstétrique, CHI de Poissy-St-Germain, Poissy, France. 5. Unité de Pathologie Cellulaire et Génétique - Equipe d'Accueil 2493, Université de Versailles-St. Quentin, Unité de Formation et de Recherche des Sciences de la Santé, Montigny le Bretonneux, France; Service de Biochimie et Génétique Moléculaire, Hôpital A. Paré, Boulogne, France.
Abstract
OBJECTIVE: To measure mitochondrial content and the expression of estrogen-related receptor-γ (ERRγ, a major inducer of mitochondrial biogenesis) in placentas from women with intrauterine growth restriction (IUGR) associated or not with pre-eclampsia (PE), relative to control placentas. DESIGN: Case-control study. SETTING: Teaching hospital and university research laboratory. PATIENT(S): Thirty-nine placentas from women with IUGR, 8 IUGR+PE, and 30 controls. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Mitochondrial DNA and protein content, gene and protein expression. RESULT(S): We observed significantly lower placental mitochondrial DNA and protein contents (associated with down-regulation of ERRγ expression) in IUGR and IUGR+PE placentas, relative to control placentas. Our results also revealed that the placental mitochondrial DNA content was directly correlated with fetal weight. Moreover, we observed significantly lower peroxisome proliferator-activated receptor-γ coactivator-1α and sirtuin 1 messenger RNA expression levels in IUGR+PE placentas, relative to control placentas. CONCLUSION(S): The low mitochondrial DNA and protein contents observed in IUGR placentas are probably due to down-regulation of ERRγ expression. This finding suggests that ERRγ has a major role in the control of placental development.
OBJECTIVE: To measure mitochondrial content and the expression of estrogen-related receptor-γ (ERRγ, a major inducer of mitochondrial biogenesis) in placentas from women with intrauterine growth restriction (IUGR) associated or not with pre-eclampsia (PE), relative to control placentas. DESIGN: Case-control study. SETTING: Teaching hospital and university research laboratory. PATIENT(S): Thirty-nine placentas from women with IUGR, 8 IUGR+PE, and 30 controls. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Mitochondrial DNA and protein content, gene and protein expression. RESULT(S): We observed significantly lower placental mitochondrial DNA and protein contents (associated with down-regulation of ERRγ expression) in IUGR and IUGR+PE placentas, relative to control placentas. Our results also revealed that the placental mitochondrial DNA content was directly correlated with fetal weight. Moreover, we observed significantly lower peroxisome proliferator-activated receptor-γ coactivator-1α and sirtuin 1 messenger RNA expression levels in IUGR+PE placentas, relative to control placentas. CONCLUSION(S): The low mitochondrial DNA and protein contents observed in IUGR placentas are probably due to down-regulation of ERRγ expression. This finding suggests that ERRγ has a major role in the control of placental development.
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