Literature DB >> 2604734

Time-dependent activity and expression of glutathione S-transferases in the human colon adenocarcinoma cell line Caco-2.

W H Peters1, H M Roelofs.   

Abstract

The human colon carcinoma cell line Caco-2 was examined for glutathione S-transferase (GST) composition and activity. Freshly seeded cells and cells until 4 days after confluency contain only the placental (Pi) form of glutathione transferase. Cells in culture for longer periods start to express class-Alpha GST isoenzymes. Confluent cells in culture for 20 days or longer contain up to 90% class-Alpha GST. Class-Mu GSTs are not detectable. GST activity gradually increases from 564 +/- 28 to 5381 +/- 165 nmol/min per mg of protein at day 0 and 32 after confluency respectively. With regard to GST composition, Caco-2 cells in culture for longer periods most resemble small-intestinal cells, whereas short-time cultures have characteristics of colonic cells. This cell line is very well suited for the study of both the in vitro properties and the expression of class Alpha and Pi GSTs.

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Year:  1989        PMID： 2604734      PMCID： PMC1133624          DOI： 10.1042/bj2640613

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  21 in total

Review 1.  The glutathione S-transferases: an update.

Authors:  T D Boyer
Journal:  Hepatology       Date:  1989-03       Impact factor: 17.425

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Expression of anionic glutathione-S-transferase and P-glycoprotein genes in human tissues and tumors.

Authors:  J A Moscow; C R Fairchild; M J Madden; D T Ransom; H S Wieand; E E O'Brien; D G Poplack; J Cossman; C E Myers; K H Cowan
Journal:  Cancer Res       Date:  1989-03-15       Impact factor: 12.701

4.  Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.

Authors:  W H Habig; M J Pabst; W B Jakoby
Journal:  J Biol Chem       Date:  1974-11-25       Impact factor: 5.157

5.  Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability.

Authors:  I J Hidalgo; T J Raub; R T Borchardt
Journal:  Gastroenterology       Date:  1989-03       Impact factor: 22.682

Review 6.  Glutathione transferases--structure and catalytic activity.

Authors:  B Mannervik; U H Danielson
Journal:  CRC Crit Rev Biochem       Date:  1988

7.  Isolation of the human anionic glutathione S-transferase cDNA and the relation of its gene expression to estrogen-receptor content in primary breast cancer.

Authors:  J A Moscow; A J Townsend; M E Goldsmith; J Whang-Peng; P J Vickers; R Poisson; S Legault-Poisson; C E Myers; K H Cowan
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

8.  The activity of the peroxide-metabolizing system in human colon carcinoma.

Authors:  G Baur; A Wendel
Journal:  J Cancer Res Clin Oncol       Date:  1980       Impact factor: 4.553

9.  Human intestinal glutathione S-transferases.

Authors:  W H Peters; H M Roelofs; F M Nagengast; J H van Tongeren
Journal:  Biochem J       Date:  1989-01-15       Impact factor: 3.857

10.  Glutathione and glutathione-dependent enzymes in ovarian adenocarcinoma cell lines derived from a patient before and after the onset of drug resistance: intrinsic differences and cell cycle effects.

Authors:  A D Lewis; J D Hayes; C R Wolf
Journal:  Carcinogenesis       Date:  1988-07       Impact factor: 4.944
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  8 in total

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Review 2.  The human intestinal epithelial cell line Caco-2; pharmacological and pharmacokinetic applications.

Authors:  V Meunier; M Bourrié; Y Berger; G Fabre
Journal:  Cell Biol Toxicol       Date:  1995-08       Impact factor: 6.691

3.  Glutathione-conjugate transport by human colon adenocarcinoma cells (Caco-2 cells).

Authors:  R P Oude Elferink; C T Bakker; P L Jansen
Journal:  Biochem J       Date:  1993-03-15       Impact factor: 3.857

4.  Antioxidant enzymes in the differentiated Caco-2 cell line.

Authors:  S S Baker; R D Baker
Journal:  In Vitro Cell Dev Biol       Date:  1992 Sep-Oct

5.  Sensing Small Changes in Protein Abundance: Stimulation of Caco-2 Cells by Human Whey Proteins.

Authors:  Judy K Cundiff; Elizabeth J McConnell; Kimberly J Lohe; Sarah D Maria; Robert J McMahon; Qiang Zhang
Journal:  J Proteome Res       Date:  2015-12-15       Impact factor: 4.466

6.  In vitro toxicity and formation of early conjugates in Caco-2 cell line treated with clenbuterol, salbutamol and isoxsuprine.

Authors:  A Stammati; P Badino; I De Angelis; G Re; O Vincentini; C Zanetti; F Zucco; G Brambilla
Journal:  Eur J Drug Metab Pharmacokinet       Date:  1997 Apr-Jun       Impact factor: 2.569

7.  Modulation of cytochrome P450 metabolism and transport across intestinal epithelial barrier by ginger biophenolics.

Authors:  Rao Mukkavilli; Sushma R Gundala; Chunhua Yang; Shashikiran Donthamsetty; Guilherme Cantuaria; Gajanan R Jadhav; Subrahmanyam Vangala; Michelle D Reid; Ritu Aneja
Journal:  PLoS One       Date:  2014-09-24       Impact factor: 3.240

8.  Drug-transporter mediated interactions between anthelminthic and antiretroviral drugs across the Caco-2 cell monolayers.

Authors:  Gabriel Kigen; Geoffrey Edwards
Journal:  BMC Pharmacol Toxicol       Date:  2017-05-04       Impact factor: 2.483
  8 in total

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