Literature DB >> 2604732

Kinetic features of ascorbic acid oxidase after partial deglycation.

G D'Andrea1, M Maccarrone, A Oratore, L Avigliano, A Messerschmidt.   

Abstract

By means of specific exoglycosidases, sugars have been removed under non-denaturing conditions from ascorbic acid oxidase (AAO), different deglycation schedules being followed. Our results indicate that deglycation clearly affects the kinetic features of AAO, leading to an increase of 'affinity' and 'catalytic ability' of the enzymic forms so generated. A better exposure of the catalytic-site residues could be supposed to occur upon treatment with exoglycosidases. This is supported by the three-dimensional X-ray structure of zucchini (Cucurbita pepo medullosa; courgette) AAO.

Entities:  

Mesh：

Substances：

Year:  1989        PMID： 2604732      PMCID： PMC1133621          DOI： 10.1042/bj2640601

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  11 in total

1.  Primary structure of cucumber (Cucumis sativus) ascorbate oxidase deduced from cDNA sequence: homology with blue copper proteins and tissue-specific expression.

Authors:  J Ohkawa; N Okada; A Shinmyo; M Takano
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

2.  X-ray crystal structure of the blue oxidase ascorbate oxidase from zucchini. Analysis of the polypeptide fold and a model of the copper sites and ligands.

Authors:  A Messerschmidt; A Rossi; R Ladenstein; R Huber; M Bolognesi; G Gatti; A Marchesini; R Petruzzelli; A Finazzi-Agró
Journal:  J Mol Biol       Date:  1989-04-05       Impact factor: 5.469

3.  Alterations in antigenic structure of gonadotropins following deglycosylation.

Authors:  M R Sairam; J Linggen; G N Bhargavi
Journal:  Biosci Rep       Date:  1988-06       Impact factor: 3.840

Review 4.  The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins.

Authors:  G Ashwell; A G Morell
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1974

Review 5.  The lipid pathway of protein glycosylation and its inhibitors: the biological significance of protein-bound carbohydrates.

Authors:  R T Schwarz; R Datema
Journal:  Adv Carbohydr Chem Biochem       Date:  1982       Impact factor: 12.200

Review 6.  Carbohydrate moieties of glycoproteins. A re-evaluation of their function.

Authors:  K Olden; J B Parent; S L White
Journal:  Biochim Biophys Acta       Date:  1982-05-12

7.  Evidence for a glycoprotein "signal" involved in transport between subcellular organelles. Two membrane glycoproteins encoded by murine leukemia virus reach the cell surface at different rates.

Authors:  T Fitting; D Kabat
Journal:  J Biol Chem       Date:  1982-12-10       Impact factor: 5.157

8.  Cathepsin D and beta-hexosaminidase synthesized in the presence of 1-deoxynojirimycin accumulate in the endoplasmic reticulum.

Authors:  P Lemansky; V Gieselmann; A Hasilik; K von Figura
Journal:  J Biol Chem       Date:  1984-08-25       Impact factor: 5.157

9.  Effect of tunicamycin, an inhibitor of protein glycosylation, on the biological properties of acetylcholine receptor in cultured muscle cells.

Authors:  J Prives; D Bar-Sagi
Journal:  J Biol Chem       Date:  1983-02-10       Impact factor: 5.157
View more
  1 in total

1.  Effect of tunicamycin on the activity and immunoreactivity of ascorbate oxidase (Cucurbita pepo medullosa) expressed in cultured green zucchini cells.

Authors:  G Pitari; G D'Andrea; M L Salucci; A Rossi; L Avigliano
Journal:  Glycoconj J       Date:  1998-08       Impact factor: 2.916
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.