Yundan Liang1, Gaofeng Zhao2, Ruifen Sun3, Yuanyi Mao4, Gangqin Li1, Xueyan Chen1, Linbo Gao5, Zeqing Hu6. 1. Department of Forensic Psychiatry, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, PR China. 2. Mental Health Hospital of Jining, Jining, Shandong 272051, PR China. 3. Central Laboratory, Yunnan University of Chinese Traditional Medicine, Kunming, Yunnan 650500, PR China. 4. Criminal Detachment of Chengdu Public Security Bureau, Chengdu, Sichuan 610017, PR China. 5. Laboratory of Molecular and Translational Medicine, West China Institute of Women and Children׳s Health; West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, PR China. Electronic address: gaolinboscu@163.com. 6. Department of Forensic Psychiatry, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, PR China. Electronic address: huzeqing@126.com.
Abstract
BACKGROUND: Let-7 family plays a critical role in the pathogenesis of major depressive disorder (MDD). Genetic polymorphisms in the promoters of miRNA may influence individual׳s susceptibility to diseases. The purpose of this study was to investigate the association between rs10877887 and rs13293512 polymorphisms in the promoters of let-7 family and the risk of MDD. METHOD: Polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing assays were used to analyze the rs10877887 and rs13293512 polymorphisms in 237 MDD patients and 296 controls. RESULTS: We found that the rs10877887 CC genotype was associated with an increased risk of MDD (CC vs. TT: OR=1.73, 95% CI, 1.04-2.86, P=0.03, and CC vs. TT/TC: OR=1.74, 95% CI, 1.08-2.80, P=0.02, respectively). Similarly increased risk was also observed for the rs13293512 (CC vs. TT: OR=1.83, 95% CI, 1.12-2.99, P=0.015; CC vs. TT/TC: OR=1.84, 95% CI, 1.20-2.81, P=0.005; and C vs. T: OR=1.32, 95% CI, 1.03-1.68, P=0.03, respectively). Stratification analysis showed that patients with the rs13293512 TC and CC genotypes had a 2.29 and 2.56-fold increased risk of MDD recurrence after treatment (TC vs. TT: 95% CI, 1.23-4.25, P=0.008; CC vs. TT: 95% CI, 1.25-5.23, P=0.009, respectively). LIMITATIONS: Relatively small sample size and hospital-based study design may influence the results. CONCLUSIONS: Our findings suggest that the rs10877887 and rs13293512 polymorphisms may be related to the development of MDD.
BACKGROUND: Let-7 family plays a critical role in the pathogenesis of major depressive disorder (MDD). Genetic polymorphisms in the promoters of miRNA may influence individual׳s susceptibility to diseases. The purpose of this study was to investigate the association between rs10877887 and rs13293512 polymorphisms in the promoters of let-7 family and the risk of MDD. METHOD: Polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing assays were used to analyze the rs10877887 and rs13293512 polymorphisms in 237 MDDpatients and 296 controls. RESULTS: We found that the rs10877887 CC genotype was associated with an increased risk of MDD (CC vs. TT: OR=1.73, 95% CI, 1.04-2.86, P=0.03, and CC vs. TT/TC: OR=1.74, 95% CI, 1.08-2.80, P=0.02, respectively). Similarly increased risk was also observed for the rs13293512 (CC vs. TT: OR=1.83, 95% CI, 1.12-2.99, P=0.015; CC vs. TT/TC: OR=1.84, 95% CI, 1.20-2.81, P=0.005; and C vs. T: OR=1.32, 95% CI, 1.03-1.68, P=0.03, respectively). Stratification analysis showed that patients with the rs13293512 TC and CC genotypes had a 2.29 and 2.56-fold increased risk of MDD recurrence after treatment (TC vs. TT: 95% CI, 1.23-4.25, P=0.008; CC vs. TT: 95% CI, 1.25-5.23, P=0.009, respectively). LIMITATIONS: Relatively small sample size and hospital-based study design may influence the results. CONCLUSIONS: Our findings suggest that the rs10877887 and rs13293512 polymorphisms may be related to the development of MDD.