Literature DB >> 2604704

Purification of TEM-1 beta-lactamase by immunoaffinity chromatography.

E Bibi1.   

Abstract

A monoclonal antibody prepared against TEM-1 beta-lactamase was found to compete with penicillins and cephalosporins for binding to the enzyme. The purified antibody preparation was linked to Sepharose 4B and used for immunoaffinity-chromatography purification of TEM-1 beta-lactamase. Elution with either benzylpenicillin or cloxacillin yielded a highly purified, concentrated and active enzyme preparation.

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Year:  1989        PMID： 2604704      PMCID： PMC1133427          DOI： 10.1042/bj2630309

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  A general method for site-specific incorporation of unnatural amino acids into proteins.

Authors:  C J Noren; S J Anthony-Cahill; M C Griffith; P G Schultz
Journal:  Science       Date:  1989-04-14       Impact factor: 47.728

2.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

3.  Substitution of tyrosine for either cysteine in beta-lactamase prevents release from the membrane during secretion.

Authors:  R Fitts; Z Reuveny; J van Amsterdam; J Mulholland; D Botstein
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

4.  Acquisition of substrate-specific parameters during the catalytic reaction of penicillinase.

Authors:  N Citri; A Samuni; N Zyk
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

5.  A direct spectrophotometric assay and determination of Michaelis constants for the beta-lactamase reaction.

Authors:  A Samuni
Journal:  Anal Biochem       Date:  1975-01       Impact factor: 3.365

6.  beta-Lactamase proceeds via an acyl-enzyme intermediate. Interaction of the Escherichia coli RTEM enzyme with cefoxitin.

Authors:  J Fisher; J G Belasco; S Khosla; J R Knowles
Journal:  Biochemistry       Date:  1980-06-24       Impact factor: 3.162

7.  Transferable resistance to third-generation cephalosporins in clinical isolates of Klebsiella pneumoniae: identification of CTX-1, a novel beta-lactamase.

Authors:  D Sirot; J Sirot; R Labia; A Morand; P Courvalin; A Darfeuille-Michaud; R Perroux; R Cluzel
Journal:  J Antimicrob Chemother       Date:  1987-09       Impact factor: 5.790

8.  Preparation of gram quantities of a purified R-factor-mediated penicillinase from Escherichia coli strain W3310.

Authors:  J Melling; G K Scott
Journal:  Biochem J       Date:  1972-11       Impact factor: 3.857

9.  Stability of wild-type and mutant RTEM-1 beta-lactamases: effect of the disulfide bond.

Authors:  S C Schultz; G Dalbadie-McFarland; J J Neitzel; J H Richards
Journal:  Proteins       Date:  1987
  9 in total
  1 in total

1.  Development of a sensitive and specific enzyme-linked immunosorbent assay for detecting and quantifying CMY-2 and SHV beta-lactamases.

Authors:  Andrea M Hujer; Malcolm G P Page; Marion S Helfand; Bethany Yeiser; Robert A Bonomo
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948
  1 in total

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