Leila Azimi1, Gholamreza Erajiyan2, Malihe Talebi2, Parviz Owlia3, Mahsa Bina2, Ali Shojaie4, Abdolaziz Rastegar Lari5. 1. Faculty, Antimicrobial Resistance Research Center, Iran University of Medical sciences, Tehran, Iran. And Department of Microbiology, Iran University of Medical sciences , Tehran, Iran . 2. Faculty, Department of Microbiology, Iran University of Medical sciences , Tehran, Iran . 3. Faculty, Molecular Microbiology Research Center, Shahed University , Tehran, Iran . 4. Faculty, Tehran Heart center, Tehran university of Medical Sciences , Tehran, Iran . 5. Faculty, Antimicrobial Resistance Research Center Iran University of Medical sciences, Tehran, Iran. And Department of Microbiology, Iran University of Medical sciences , Tehran, Iran .
Abstract
INTRODUCTION: Klebsiella pneumoniae is one of the main opportunistic pathogens which can cause different types of infections. Production of beta-lactamases like AmpC and ESBL mostly lead to beta-lactam resistance in these Gram-Negative bacteria. The aim of this study was the detection of AmpC-producing K. pneumoniae in clinical isolates. MATERIALS AND METHODS: Three hundred and three isolates of K. pneumoniae were identified. Double disc method including cefoxitin with cefepime and using boronic acid with cloxacillin were performed as two phenotypic methods for detection of AmpC. Amplification of AmpC gene was performed by PCR. RESULTS: Eight and three isolates showed positive results in double disc method and by using boronic acid with cloxacillin, respectively. Five isolates had specific band for AmpC gene after electrophoresis. CONCLUSION: Our results were indicated the low prevalence of AmpC-producer-K. pnemoniae in Iran. On the other hand these two tested phenotypic methods showed low sensitivity for detection of AmpC.
INTRODUCTION:Klebsiella pneumoniae is one of the main opportunistic pathogens which can cause different types of infections. Production of beta-lactamases like AmpC and ESBL mostly lead to beta-lactam resistance in these Gram-Negative bacteria. The aim of this study was the detection of AmpC-producing K. pneumoniae in clinical isolates. MATERIALS AND METHODS: Three hundred and three isolates of K. pneumoniae were identified. Double disc method including cefoxitin with cefepime and using boronic acid with cloxacillin were performed as two phenotypic methods for detection of AmpC. Amplification of AmpC gene was performed by PCR. RESULTS: Eight and three isolates showed positive results in double disc method and by using boronic acid with cloxacillin, respectively. Five isolates had specific band for AmpC gene after electrophoresis. CONCLUSION: Our results were indicated the low prevalence of AmpC-producer-K. pnemoniae in Iran. On the other hand these two tested phenotypic methods showed low sensitivity for detection of AmpC.
Authors: Lalitagauri M Deshpande; Ronald N Jones; Thomas R Fritsche; Helio S Sader Journal: Int J Antimicrob Agents Date: 2006-11-16 Impact factor: 5.283
Authors: Enrique Rodríguez-Guerrero; Juan Carlos Callejas-Rodelas; José María Navarro-Marí; José Gutiérrez-Fernández Journal: Microorganisms Date: 2022-03-14