Weiguo Wang1, Zhihong Guo2, Zhanwang Xu3, Qingxi Meng4, Chen Chen1, Yaoguang Zhang5, Xuecheng Cao1. 1. Department of Orthopaedic Surgery, General Hospital of Jinan Military Command Jinan 250031, China. 2. Department of Anesthesiology, General Hospital of Jinan Military Command Jinan 250031, China. 3. Department of Orthopaedics, The First Affiliated Hospital of Shandong University of Traditional Chinese Medicine Jinan 250014, China. 4. Department of Spinal Cord Repair, General Hospital of Jinan Military Command Jinan 250031, China. 5. Department of Emergency Surgery, General Hospital of Jinan Military Command Jinan 250031, China.
Abstract
PURPOSE: To investigate the effects and mechanism of pollen typhae on spinal cord injury (SCI) in rats. METHODS: The SCI model was built and animals were randomly divided into three groups according to different concentrations of pollen typhae. Protein, mRNA, and fluorescence expression levels of light-chain-3 (LC-3) and Beclin-1 were determined by western blotting (WB), real-time PCR and immunofluorescence, along as Akt and mammalian target of rapamycin (mROT) by WB. The demyelination area and integrated optical density (IOD) were analyzed by luxol fast blue (LFB) and Nissl staining, respectively; Behavioral assessments were assessed by Basso Beattie Bresnahan (BBB) scale. RESULTS: Protein, mRNA, and fluorescence expression levels of LC-3 and Beclin-1 were significantly increased after SCI, while were obviously decreased by administration of pollen typhae, along with protein level of Akt and mROT. The demyelination area was significantly reduced, while IOD and BBB were significantly increased compared with the model group. CONCLUSION: Autophagic activity increased in damaged neural tissue after SCI, and pollen typhae have certain therapeutic effect on SCI, the higher concentration of pollen typhae, the more effective. Besides, pollen typhae also provided neuroprotective effect and improved locomotor function. The effects may be produced by blockade of Akt/mTOR pathway.
PURPOSE: To investigate the effects and mechanism of pollen typhae on spinal cord injury (SCI) in rats. METHODS: The SCI model was built and animals were randomly divided into three groups according to different concentrations of pollen typhae. Protein, mRNA, and fluorescence expression levels of light-chain-3 (LC-3) and Beclin-1 were determined by western blotting (WB), real-time PCR and immunofluorescence, along as Akt and mammalian target of rapamycin (mROT) by WB. The demyelination area and integrated optical density (IOD) were analyzed by luxol fast blue (LFB) and Nissl staining, respectively; Behavioral assessments were assessed by Basso Beattie Bresnahan (BBB) scale. RESULTS: Protein, mRNA, and fluorescence expression levels of LC-3 and Beclin-1 were significantly increased after SCI, while were obviously decreased by administration of pollen typhae, along with protein level of Akt and mROT. The demyelination area was significantly reduced, while IOD and BBB were significantly increased compared with the model group. CONCLUSION: Autophagic activity increased in damaged neural tissue after SCI, and pollen typhae have certain therapeutic effect on SCI, the higher concentration of pollen typhae, the more effective. Besides, pollen typhae also provided neuroprotective effect and improved locomotor function. The effects may be produced by blockade of Akt/mTOR pathway.