| Literature DB >> 26045735 |
Wei Li1, Dayong Du1, Hang Wang2, Yang Liu1, Xiaohui Lai1, Feng Jiang1, Dong Chen1, Yanbin Zhang1, Jiaxin Zong1, Yuntian Li1.
Abstract
Silent information regulator 1 (SIRT1) mediates many effects of caloric restriction (CR) on an organism's lifespan and metabolic pathways. Recent reports have also emphasized its role in vascular function. The present study was designed to investigate the effects of SIRT1 on the properties of mouse spleen derived endothelial progenitor cells (EPCs). SIRT1 in EPCs was significantly increased by serum and by vascular endothelial growth factor (VEGF). Moreover, an adenovirus (Ad) vector expressing SIRT1 (Ad-SIRT1)-mediated overexpression of SIRT1 directly enhanced migration and proliferation of EPCs, whereas silencing of endogenous SIRT1 in EPCs inhibited cell functions. In addition, LY294002 (a PI3K inhibitor), sc-221226 (an Akt inhibitor), and L-NAME (an NOS inhibitor) abolished Ad-SIRT1-induced migration and proliferation of EPCs, and prevented nitric oxide (NO) production. Phosphorylation of Akt, PI3K, and endothelial nitricoxide synthase (eNOS) were up-regulated by Ad-SIRT1, which was attenuated by LY294002, sc-221226, and L-NAME. Together, the results suggested that through the PI3K/Akt/eNOS signaling pathway, SIRT1 plays an important role in the biological properties of EPCs.Entities:
Keywords: Silent information regulator 1; endothelial progenitor cells; migration; proliferation
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Year: 2015 PMID： 26045735 PMCID： PMC4440044
Source DB: PubMed Journal: Int J Clin Exp Pathol ISSN： 1936-2625