Jérôme Delroisse1, Olga Ortega-Martinez2, Sam Dupont3, Jérôme Mallefet4, Patrick Flammang5. 1. University of Mons - UMONS, Research Institute for Biosciences, Biology of Marine Organisms and Biomimetics, 23 Place du Parc, 7000 Mons, Belgium. Electronic address: jerome.delroisse@umons.ac.be. 2. University of Gothenburg, Department of Biological and Environmental Science, The Sven Lovén Centre for Marine Sciences, Kristineberg, 45178 Fiskebäckskil, Sweden. Electronic address: olga.ortega-martinez@bioenv.gu.se. 3. University of Gothenburg, Department of Biological and Environmental Science, The Sven Lovén Centre for Marine Sciences, Kristineberg, 45178 Fiskebäckskil, Sweden. Electronic address: sam.dupont@bioenv.gu.se. 4. Catholic University of Louvain-La-Neuve, Marine Biology Laboratory, Place croix du Sud, Louvain-La-Neuve, Belgium. Electronic address: jerome.mallefet@uclouvain.be. 5. University of Mons - UMONS, Research Institute for Biosciences, Biology of Marine Organisms and Biomimetics, 23 Place du Parc, 7000 Mons, Belgium. Electronic address: patrick.flammang@umons.ac.be.
Abstract
BACKGROUND: In non-classical model species, Next Generation Sequencing increases the ability to analyze the expression of transcripts/genes. In this study, paired-end Illumina HiSeq sequencing technology has been employed to describe a larval transcriptome generated from 64 h post-fertilization pluteus larvae of the brittle star Amphiura filiformis. We focused our analysis on the detection of actors involved in the opsin based light perception, respectively the opsins and the phototransduction actors. METHODS & RESULTS: In this research, about 47 million high quality reads were generated and 86,572 total unigenes were predicted after de novo assembly. Of all the larval unigenes, 18% show significant matches with reference online databases. 46% of annotated larval unigenes were significantly similar to transcripts from the purple sea urchin. COG, GO and KEGG analyses were performed on predicted unigenes. Regarding the opsin-based photoreception process, even if possible actors of ciliary and rhabdomeric phototransduction cascades were detected, no ciliary or rhabdomeric opsin was identified in these larvae. Additionally, partial non-visual RGR (retinal G protein coupled receptor) opsin mRNAs were identified,possibly indicating the presence of visual cycle reaction in early pluteus larvae. The eye morphogene Pax 6 was also identified in the pluteus transcriptome. CONCLUSIONS: Contrary to sea-urchin larvae, brittle star larvae appear to be characterized by an absence of visual-like opsins. These RNA-seq data also provide a useful resource for the echinoderm research community and researchers with an interest in larval biology.
BACKGROUND: In non-classical model species, Next Generation Sequencing increases the ability to analyze the expression of transcripts/genes. In this study, paired-end Illumina HiSeq sequencing technology has been employed to describe a larval transcriptome generated from 64 h post-fertilization pluteus larvae of the brittle star Amphiura filiformis. We focused our analysis on the detection of actors involved in the opsin based light perception, respectively the opsins and the phototransduction actors. METHODS & RESULTS: In this research, about 47 million high quality reads were generated and 86,572 total unigenes were predicted after de novo assembly. Of all the larval unigenes, 18% show significant matches with reference online databases. 46% of annotated larval unigenes were significantly similar to transcripts from the purple sea urchin. COG, GO and KEGG analyses were performed on predicted unigenes. Regarding the opsin-based photoreception process, even if possible actors of ciliary and rhabdomeric phototransduction cascades were detected, no ciliary or rhabdomeric opsin was identified in these larvae. Additionally, partial non-visual RGR (retinal G protein coupled receptor) opsin mRNAs were identified,possibly indicating the presence of visual cycle reaction in early pluteus larvae. The eye morphogene Pax 6 was also identified in the pluteus transcriptome. CONCLUSIONS: Contrary to sea-urchin larvae, brittle star larvae appear to be characterized by an absence of visual-like opsins. These RNA-seq data also provide a useful resource for the echinoderm research community and researchers with an interest in larval biology.
Authors: Meet Zandawala; Ismail Moghul; Luis Alfonso Yañez Guerra; Jérôme Delroisse; Nikara Abylkassimova; Andrew F Hugall; Timothy D O'Hara; Maurice R Elphick Journal: Open Biol Date: 2017-09 Impact factor: 6.411
Authors: Jessica A Goodheart; Geetanjali Minsky; Mira N Brynjegard-Bialik; Michael S Drummond; J David Munoz; Timothy R Fallon; Darrin T Schultz; Jing-Ke Weng; Elizabeth Torres; Todd H Oakley Journal: Sci Rep Date: 2020-06-26 Impact factor: 4.379