| Literature DB >> 26044023 |
Hemangi B Shah1, Kristi A Koelsch.
Abstract
The B-cell ELISPOT assay is a sensitive tool that can be utilized to measure total immunoglobulin (Ig) and antigen-specific antibody-secreting cells. Typically, membrane-bound antigen enables binding of antibody secreted by B-cells. Bound antibody is then detected by using an anti-Ig antibody and a colorimetric substrate, resulting in colored spots on the membrane that can be easily enumerated. Here we have described a method to quantitate antigen-specific antibody-secreting cells from the spleen or bone marrow of a vaccinated mouse.Entities:
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Year: 2015 PMID： 26044023 DOI： 10.1007/978-1-4939-2694-7_42
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745