Literature DB >> 26043986

Western blotting: an introduction.

Biji T Kurien1, R Hal Scofield.   

Abstract

Western blotting is an important procedure for the immunodetection of proteins, particularly proteins that are of low abundance. This process involves the transfer of protein patterns from gel to microporous membrane. Electrophoretic as well as non-electrophoretic transfer of proteins to membranes was first described in 1979. Protein blotting has evolved greatly since the inception of this protocol, allowing protein transfer to be accomplished in a variety of ways.

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Year:  2015        PMID： 26043986      PMCID： PMC7304528          DOI： 10.1007/978-1-4939-2694-7_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  46 in total

1.  Identification of glycoproteins on nitrocellulose membranes and gels.

Authors:  D J Thornton; I Carlstedt; J K Sheehan
Journal:  Mol Biotechnol       Date:  1996-04       Impact factor: 2.695

2.  High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  M W Bolt; P A Mahoney
Journal:  Anal Biochem       Date:  1997-05-01       Impact factor: 3.365

3.  Checkerboard immunoblotting (CBIB): an efficient, rapid, and sensitive method of assaying multiple antigen/antibody cross-reactivities.

Authors:  M Kazemi; R A Finkelstein
Journal:  J Immunol Methods       Date:  1990-03-27       Impact factor: 2.303

Review 4.  Protein blotting: a manual.

Authors:  J M Gershoni
Journal:  Methods Biochem Anal       Date:  1988

5.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors:  J C Alwine; D J Kemp; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

6.  Protein transfer from isoelectric focusing Gels: the native blot.

Authors:  M P Reinhart; D Malamud
Journal:  Anal Biochem       Date:  1982-07-01       Impact factor: 3.365

7.  "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors:  W N Burnette
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

8.  Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.

Authors:  J Renart; J Reiser; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

9.  Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membrane.

Authors:  B T Kurien; H Matsumoto; R H Scofield
Journal:  Indian J Biochem Biophys       Date:  2001-08       Impact factor: 1.918

10.  The phosphorylation of ribosomal protein S6 in rat tissues following cycloheximide injection, in diabetes, and after denervation of diaphragm. A simple immunological determination of the extent of S6 phosphorylation on protein blots.

Authors:  P J Nielsen; K L Manchester; H Towbin; J Gordon; G Thomas
Journal:  J Biol Chem       Date:  1982-10-25       Impact factor: 5.157
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  40 in total

1.  Small molecule target identification using photo-affinity chromatography.

Authors:  Seung-Yong Seo; Timothy W Corson
Journal:  Methods Enzymol       Date:  2019-03-15       Impact factor: 1.600

2.  Alternative Splicing of Heat Shock Transcription Factor 2 Regulates the Expression of Laccase Gene Family in Response to Copper in Trametes trogii.

Authors:  Yu Zhang; Yuanyuan Wu; Xulei Yang; En Yang; Huini Xu; Yuhui Chen; Irbis Chagan; Jinping Yan
Journal:  Appl Environ Microbiol       Date:  2021-02-12       Impact factor: 4.792

3.  Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation.

Authors:  Simone Sidoli; Sophie Trefely; Benjamin A Garcia; Alessandro Carrer
Journal:  Methods Mol Biol       Date:  2019

4.  Zinc Deficiency Exacerbates Bisphenol A-Induced Hepatic and Renal Damage: Delineation of Molecular Mechanisms.

Authors:  Aarzoo Charaya; Chittaranjan Sahu; Shivani Singla; Gopabandhu Jena
Journal:  Biol Trace Elem Res       Date:  2022-09-08       Impact factor: 4.081

5.  Inhibition of N-Acetyltransferase 10 Suppresses the Progression of Prostate Cancer through Regulation of DNA Replication.

Authors:  Ningning Ma; Haijing Liu; Yaqian Wu; Mengfei Yao; Bo Zhang
Journal:  Int J Mol Sci       Date:  2022-06-12       Impact factor: 6.208

6.  HrpA anchors meningococci to the dynein motor and affects the balance between apoptosis and pyroptosis.

Authors:  Adelfia Talà; Flora Guerra; Matteo Calcagnile; Roberta Romano; Silvia Caterina Resta; Aurora Paiano; Mario Chiariello; Graziano Pizzolante; Cecilia Bucci; Pietro Alifano
Journal:  J Biomed Sci       Date:  2022-06-28       Impact factor: 12.771

7.  [Escin alleviates chemotherapy-induced peripheral neuropathic pain by inducing autophagy in the spinal cord of rats].

Authors:  Fang Yan; Dongtai Chen; Jingdun Xie; Weian Zeng; Qiang Li
Journal:  Nan Fang Yi Ke Da Xue Xue Bao       Date:  2020-11-30

8.  Comparison of the sensitivity of Western blotting between PVDF and NC membranes.

Authors:  Yufang Xiang; Yuanyuan Zheng; Shaobo Liu; Gang Liu; Zhi Li; Weijie Dong
Journal:  Sci Rep       Date:  2021-06-08       Impact factor: 4.379

9.  Long non-coding RNA BBOX1-antisense RNA 1 enhances cell proliferation and migration and suppresses apoptosis in oral squamous cell carcinoma via the miR-3940-3p/laminin subunit gamma 2 axis.

Authors:  Chunguang Zhao; Wei Shi; Min Chen
Journal:  Bioengineered       Date:  2022-04       Impact factor: 6.832

10.  Curcumin Induces Autophagy, Apoptosis, and Cell Cycle Arrest in Human Pancreatic Cancer Cells.

Authors:  Yaping Zhu; Shurui Bu
Journal:  Evid Based Complement Alternat Med       Date:  2017-09-10       Impact factor: 2.629
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