Lewis Fall1, Karl J New1, Kevin A Evans1, Damian M Bailey1. 1. Faculty of Computing, Engineering and Science, Neurovascular Research Laboratory, Research Institute of Science and Health, University of South Wales, Glyntaff, UK.
Abstract
AIMS: Arterial hypoxaemia stimulates free radical formation. Cellular studies suggest this may be implicated in coagulation activation though human evidence is lacking. To examine this, an observational study was designed to explore relationships between systemic oxidative stress and haemostatic responses in healthy participants exposed to inspiratory hypoxia. RESULTS: Activated partial thromboplastin time and international normalised ratio were measured as routine clinical biomarkers of coagulation and ascorbate free radical (A(•-)) as a direct global biomarker of free radical flux. Six hours of hypoxia activated coagulation, and increased formation of A(•-), with inverse correlations observed against oxyhaemoglobin saturation. CONCLUSIONS: This is the first study to address the link between free radical formation and coagulation in vivo. This 'proof-of-concept' study demonstrated functional associations between hypoxaemia and coagulation that may be subject to redox activation of the intrinsic pathway. Further studies are required to identify precisely which intrinsic factors are subject to redox activation. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.
AIMS: Arterial hypoxaemia stimulates free radical formation. Cellular studies suggest this may be implicated in coagulation activation though human evidence is lacking. To examine this, an observational study was designed to explore relationships between systemic oxidative stress and haemostatic responses in healthy participants exposed to inspiratory hypoxia. RESULTS: Activated partial thromboplastin time and international normalised ratio were measured as routine clinical biomarkers of coagulation and ascorbate free radical (A(•-)) as a direct global biomarker of free radical flux. Six hours of hypoxia activated coagulation, and increased formation of A(•-), with inverse correlations observed against oxyhaemoglobin saturation. CONCLUSIONS: This is the first study to address the link between free radical formation and coagulation in vivo. This 'proof-of-concept' study demonstrated functional associations between hypoxaemia and coagulation that may be subject to redox activation of the intrinsic pathway. Further studies are required to identify precisely which intrinsic factors are subject to redox activation. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.
Authors: Lewis Fall; Julien V Brugniaux; Danielle Davis; Christopher J Marley; Bruce Davies; Karl J New; Jane McEneny; Ian S Young; Damian M Bailey Journal: J Physiol Date: 2018-08-29 Impact factor: 5.182
Authors: Takuro Washio; Benjamin S Stacey; Shigehiko Ogoh; Hayato Tsukamoto; Angelo Iannetelli; Thomas S Owens; Thomas A Calverley; Lewis Fall; Christopher J Marley; Damian M Bailey Journal: J Physiol Sci Date: 2022-07-20 Impact factor: 2.257