| Literature DB >> 26042795 |
Xiaoping Bao1, Xiaojun Lian1,2, Kaitlin K Dunn1, Mengxuan Shi1, Tianxiao Han1, Tongcheng Qian1, Vijesh J Bhute1, Scott G Canfield1, Sean P Palecek1.
Abstract
Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research and therapeutic revascularization. Here, we report a completely defined endothelial progenitor differentiation platform that uses a minimalistic medium consisting of Dulbecco's modified eagle medium and ascorbic acid, lacking of albumin and growth factors. Following hPSC treatment with a GSK-3β inhibitor and culture in this medium, this protocol generates more than 30% multipotent CD34+ CD31+ endothelial progenitors that can be purified to >95% CD34+ cells via magnetic activated cell sorting (MACS). These CD34+ progenitors are capable of differentiating into endothelial cells in serum-free inductive media. These hPSC-derived endothelial cells express key endothelial markers including CD31, VE-cadherin, and von Willebrand factor (vWF), exhibit endothelial-specific phenotypes and functions including tube formation and acetylated low-density lipoprotein (Ac-LDL) uptake. This fully defined platform should facilitate production of proliferative, xeno-free endothelial progenitor cells for both research and clinical applications.Entities:
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Year: 2015 PMID： 26042795 PMCID： PMC4516678 DOI： 10.1016/j.scr.2015.05.004
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020