Literature DB >> 26034307

Terminal Deoxynucleotidyl Transferase (TdT)-Mediated dUTP Nick-End Labeling (TUNEL) for Detection of Apoptotic Cells in Drosophila.

Donna Denton1, Sharad Kumar1.   

Abstract

A characteristic feature of apoptosis is DNA fragmentation. This fragmentation can be detected by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) of DNA in dying cells. Here, we present a protocol for TUNEL detection of apoptosis in Drosophila larval tissue, but these techniques can be adapted for other tissues and developmental stages.
© 2015 Cold Spring Harbor Laboratory Press.

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Year:  2015        PMID: 26034307     DOI: 10.1101/pdb.prot086199

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN: 1559-6095


  5 in total

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Authors:  Sarah M Crews; W Tyler McCleery; M Shane Hutson
Journal:  Dev Dyn       Date:  2015-11-16       Impact factor: 3.780

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Review 4.  Recent Advances in Multicellular Tumor Spheroid Generation for Drug Screening.

Authors:  Kwang-Ho Lee; Tae-Hyung Kim
Journal:  Biosensors (Basel)       Date:  2021-11-11

5.  Triclosan Demonstrates Synergic Effect with Amphotericin B and Fluconazole and Induces Apoptosis-Like Cell Death in Cryptococcus neoformans.

Authors:  Elaheh Movahed; Grace Min Yi Tan; Komathy Munusamy; Tee Cian Yeow; Sun Tee Tay; Won Fen Wong; Chung Yeng Looi
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  5 in total

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