Mikkel H Vendelbo1, Britt Christensen2, Solbritt B Grønbæk3, Morten Høgild3, Michael Madsen2, Steen B Pedersen3, Jens O L Jørgensen3, Niels Jessen2, Niels Møller3. 1. Departments of Endocrinology and Internal MedicineNuclear Medicine and PET CenterResearch Laboratory for Biochemical PathologyAarhus University Hospital, Nørrebrogade 44, 8000 Aarhus, Denmark Departments of Endocrinology and Internal MedicineNuclear Medicine and PET CenterResearch Laboratory for Biochemical PathologyAarhus University Hospital, Nørrebrogade 44, 8000 Aarhus, Denmark Mikkel.Vendelbo@ki.au.dk. 2. Departments of Endocrinology and Internal MedicineNuclear Medicine and PET CenterResearch Laboratory for Biochemical PathologyAarhus University Hospital, Nørrebrogade 44, 8000 Aarhus, Denmark Departments of Endocrinology and Internal MedicineNuclear Medicine and PET CenterResearch Laboratory for Biochemical PathologyAarhus University Hospital, Nørrebrogade 44, 8000 Aarhus, Denmark. 3. Departments of Endocrinology and Internal MedicineNuclear Medicine and PET CenterResearch Laboratory for Biochemical PathologyAarhus University Hospital, Nørrebrogade 44, 8000 Aarhus, Denmark.
Abstract
OBJECTIVE: Fasting and exercise stimulates, whereas glucose suppresses GH secretion, but it is uncertain how these conditions impact GH signaling in peripheral tissues. To test the original 'feast and famine hypothesis' by Rabinowitz and Zierler, according to which the metabolic effects of GH are predominant during fasting, we specifically hypothesized that fasting and exercise act in synergy to increase STAT-5b target gene expression. DESIGN AND METHODS: Eight healthy men were studied on two occasions in relation to a 1 h exercise bout: i) with a concomitant i.v. glucose infusion ('feast') and ii) after a 36 h fast ('famine'). Muscle and fat biopsy specimens were obtained before, immediately after, and 30 min after exercise. RESULTS: GH increased during exercise on both examination days and this effect was amplified by fasting, and free fatty acid (FFA) levels increased after fasting. STAT-5b phosphorylation increased similarly following exercise on both occasions. In adipose tissue, suppressors of cytokine signaling 1 (SOCS1) and SOCS2 were increased after exercise on the fasting day and both fasting and exercise increased cytokine inducible SH2-containing protein (CISH). In muscle, SOCS2 and CISH mRNA were persistently increased after fasting. Muscle SOCS1, SOCS3, and CISH mRNA expression increased, whereas SOCS2 decreased after exercise on both examination days. CONCLUSIONS: This study demonstrates that fasting and exercise act in tandem to amplify STAT-5b target gene expression (SOCS and CISH) in adipose and muscle tissue in accordance with the 'feast and famine hypothesis'; the adipose tissue signaling responses, which hitherto have not been scrutinized, may play a particular role in promoting FFA mobilization.
OBJECTIVE: Fasting and exercise stimulates, whereas glucose suppresses GH secretion, but it is uncertain how these conditions impact GH signaling in peripheral tissues. To test the original 'feast and famine hypothesis' by Rabinowitz and Zierler, according to which the metabolic effects of GH are predominant during fasting, we specifically hypothesized that fasting and exercise act in synergy to increase STAT-5b target gene expression. DESIGN AND METHODS: Eight healthy men were studied on two occasions in relation to a 1 h exercise bout: i) with a concomitant i.v. glucose infusion ('feast') and ii) after a 36 h fast ('famine'). Muscle and fat biopsy specimens were obtained before, immediately after, and 30 min after exercise. RESULTS: GH increased during exercise on both examination days and this effect was amplified by fasting, and free fatty acid (FFA) levels increased after fasting. STAT-5b phosphorylation increased similarly following exercise on both occasions. In adipose tissue, suppressors of cytokine signaling 1 (SOCS1) and SOCS2 were increased after exercise on the fasting day and both fasting and exercise increased cytokine inducible SH2-containing protein (CISH). In muscle, SOCS2 and CISH mRNA were persistently increased after fasting. Muscle SOCS1, SOCS3, and CISH mRNA expression increased, whereas SOCS2 decreased after exercise on both examination days. CONCLUSIONS: This study demonstrates that fasting and exercise act in tandem to amplify STAT-5b target gene expression (SOCS and CISH) in adipose and muscle tissue in accordance with the 'feast and famine hypothesis'; the adipose tissue signaling responses, which hitherto have not been scrutinized, may play a particular role in promoting FFA mobilization.
Authors: B B Pinkhasov; V G Selyatitskaya; D A Deev; O I Kuzminova; E L Astrakhantseva Journal: Acta Endocrinol (Buchar) Date: 2019 Jul-Sep Impact factor: 0.877
Authors: Astrid Johannesson Hjelholt; Kevin Y Lee; Mai Christiansen Arlien-Søborg; Steen Bønløkke Pedersen; John J Kopchick; Vishwajeet Puri; Niels Jessen; Jens Otto L Jørgensen Journal: Mol Metab Date: 2019-08-20 Impact factor: 7.422