| Literature DB >> 26033882 |
Thomas Mørch Frøsig1,2,3, Jiawei Yap4,5, Tina Seremet1, Rikke Lyngaa1,3, Inge Marie Svane1,6, Per Thor Straten1, Mirjam H M Heemskerk7, Gijsbert M Grotenbreg3,4, Sine Reker Hadrup1,3.
Abstract
We designed conditional ligands restricted to HLA-B*08:01, -B*35:01, and -B*44:05 and proved the use of a conditional ligand previously designed for HLA-B*15:02 together with HLA-B*15:01. Furthermore, we compared the detection capabilities of specific HLA-B*15:01-restricted T cells using the HLA-B*15:01 and HLA-B*15:02 major histocompatibility complex (MHC) multimers and found remarkable differences in the staining patterns detected by flow cytometry. These new conditional ligands greatly add to the application of MHC-based technologies in the analyses of T-cell recognition as they represent frequently expressed HLA-B molecules. This expansion of conditional ligands is important to allow T-cell detection over a wide range of HLA restrictions, and provide comprehensive understanding of the T-cell recognition in a given context.Entities:
Keywords: CD8 T cells; HLA-B molecules; MHC multimer; conditional ligands; flow cytometry
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Year: 2015 PMID： 26033882 DOI： 10.1002/cyto.a.22689
Source DB: PubMed Journal: Cytometry A ISSN： 1552-4922 Impact factor: 4.355