Piotr Widlak1, Karol Jelonek2, Anna Wojakowska2, Monika Pietrowska2, Joanna Polanska3, Łukasz Marczak4, Leszek Miszczyk2, Krzysztof Składowski2. 1. Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland. Electronic address: widlak@io.gliwice.pl. 2. Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland. 3. Institute of Automatics Control, Silesian University of Technology, Gliwice, Poland. 4. Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Poznan, Poland.
Abstract
PURPOSE: Ionizing radiation affects the proteome of irradiated cells and tissue, yet data concerning changes induced during radiation therapy (RT) in human blood are fragmentary and inconclusive. We aimed to identify features of serum proteome and associated processes involved in response to partial body irradiation during cancer treatment. METHODS AND MATERIALS: Twenty patients with head and neck squamous cell cancer (HNSCC) and 20 patients with prostate cancer received definitive intensity modulated RT. Blood samples were collected before RT, just after RT, and 1 month after the end of RT. Complete serum proteome was analyzed in individual samples, using a shotgun liquid chromatography-tandem mass spectrometry approach which allowed identification of approximately 450 proteins. Approximately 100 unique proteins were quantified in all samples after exclusion of immunoglobulins, and statistical significance of differences among consecutive samples was assessed. Processes associated with quantified proteins and their functional interactions were predicted using gene ontology tools. RESULTS: RT-induced changes were marked in the HNSCC patient group: 22 upregulated and 33 downregulated proteins were detected in post-RT sera. Most of the changes reversed during follow-up, yet levels of some proteins remained affected 1 month after the end of RT. RT-upregulated proteins were associated with acute phase, inflammatory response, and complement activation. RT-downregulated proteins were associated with transport and metabolism of lipids (plasma apolipoproteins) and blood coagulation. RT-induced changes were much weaker in prostate cancer patients, which corresponded to differences in acute radiation toxicity observed in both groups. Nevertheless, general patterns of RT-induced sera proteome changes were similar in both of the groups of cancer patients. CONCLUSIONS: In this pilot study, we proposed to identify a molecular signature of radiation response, based on specific features of serum proteome. The signature included upregulation of factors involved in acute or inflammatory response but also downregulation of plasma apolipoproteins and factors involved in blood coagulation.
PURPOSE:Ionizing radiation affects the proteome of irradiated cells and tissue, yet data concerning changes induced during radiation therapy (RT) in human blood are fragmentary and inconclusive. We aimed to identify features of serum proteome and associated processes involved in response to partial body irradiation during cancer treatment. METHODS AND MATERIALS: Twenty patients with head and neck squamous cell cancer (HNSCC) and 20 patients with prostate cancer received definitive intensity modulated RT. Blood samples were collected before RT, just after RT, and 1 month after the end of RT. Complete serum proteome was analyzed in individual samples, using a shotgun liquid chromatography-tandem mass spectrometry approach which allowed identification of approximately 450 proteins. Approximately 100 unique proteins were quantified in all samples after exclusion of immunoglobulins, and statistical significance of differences among consecutive samples was assessed. Processes associated with quantified proteins and their functional interactions were predicted using gene ontology tools. RESULTS: RT-induced changes were marked in the HNSCCpatient group: 22 upregulated and 33 downregulated proteins were detected in post-RT sera. Most of the changes reversed during follow-up, yet levels of some proteins remained affected 1 month after the end of RT. RT-upregulated proteins were associated with acute phase, inflammatory response, and complement activation. RT-downregulated proteins were associated with transport and metabolism of lipids (plasma apolipoproteins) and blood coagulation. RT-induced changes were much weaker in prostate cancerpatients, which corresponded to differences in acute radiation toxicity observed in both groups. Nevertheless, general patterns of RT-induced sera proteome changes were similar in both of the groups of cancerpatients. CONCLUSIONS: In this pilot study, we proposed to identify a molecular signature of radiation response, based on specific features of serum proteome. The signature included upregulation of factors involved in acute or inflammatory response but also downregulation of plasma apolipoproteins and factors involved in blood coagulation.
Authors: Weiliang Huang; Jianshi Yu; Tian Liu; Gregory Tudor; Amy E Defnet; Stephanie Zalesak; Praveen Kumar; Catherine Booth; Ann M Farese; Thomas J MacVittie; Maureen A Kane Journal: Health Phys Date: 2020-11 Impact factor: 2.922
Authors: Weiliang Huang; Jianshi Yu; Tian Liu; Amy E Defnet; Stephanie Zalesak; Ann M Farese; Thomas J MacVittie; Maureen A Kane Journal: Health Phys Date: 2020-11 Impact factor: 2.922
Authors: Michael D Ward; Ernst E Brueggemann; Tara Kenny; Raven E Reitstetter; Christopher R Mahone; Sylvia Trevino; Kelly Wetzel; Ginger C Donnelly; Cary Retterer; Robert B Norgren; Rekha G Panchal; Travis K Warren; Sina Bavari; Lisa H Cazares Journal: Clin Proteomics Date: 2019-02-07 Impact factor: 3.988
Authors: B Frey; J Mika; K Jelonek; L Cruz-Garcia; C Roelants; I Testard; N Cherradi; K Lumniczky; S Polozov; A Napieralska; P Widlak; U S Gaipl; C Badie; J Polanska; S M Candéias Journal: Strahlenther Onkol Date: 2020-06-09 Impact factor: 3.621
Authors: Karol Jelonek; Aleksandra Krzywon; Katarzyna Papaj; Pawel Polanowski; Krzysztof Szczepanik; Krzysztof Skladowski; Piotr Widlak Journal: Strahlenther Onkol Date: 2021-06-29 Impact factor: 3.621