| Literature DB >> 26028235 |
T O Abakumova1, A A Kuz'kina, M E Zharova, M V Zharova, D A Pozdeeva, I L Gubskii, I I Shepeleva, O M Antonova, N V Nukolova, Z I Kekelidze, V P Chekhonin.
Abstract
To study demyelination and remyelination processes and their response to different drugs, a protocol for modeling multiple sclerosis using the copper chelator cuprizone was developed. Magnetic resonance imaging confirmed the presence of demyelination lesions on week 4 of 0.6% cuprizone-containing diet. Immunohistochemical staining with polyclonal antibodies to glial fibrillary acidic protein (pAb GFAP) confirmed the increase in the number of reactive astrocytes on week 4 of diet and during remyelination (week 2 after diet). Analysis of neurophysiological functions in mice with cuprizone-induced demyelination revealed motor and behavioral deficits. This model can be used as a tool for preclinical studies of the efficiency of multiple sclerosis diagnostic and therapy.Entities:
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Year: 2015 PMID： 26028235 DOI： 10.1007/s10517-015-2903-z
Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804