Marcin Kos1, Adam Junka2, Danuta Smutnicka3, Patrycja Szymczyk4, Karolina Gluza5, Marzenna Bartoszewicz6. 1. Department of Maxillofacial and Plastic Surgery, Klinikum Oldenburg, (Head: PD Dr. med, Dr. med. dent. Lei Li), Rachel-Straus-Strasse 10, 26 133 Oldenburg, Germany. Electronic address: mkos@poczta.onet.pl. 2. Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wroclaw, (Head: Dr hab. n. med. Marzenna Bartoszewicz), Borowska 211a 4, 50 556 Wroclaw, Poland. Electronic address: feliks.junka@gmail.com. 3. Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wroclaw, (Head: Dr hab. n. med. Marzenna Bartoszewicz), Borowska 211a 4, 50 556 Wroclaw, Poland. Electronic address: danuta.ruranska-smutnicka@am.wroc.pl. 4. Mechanics Department of Wroclaw University of Technology, The Centre for Advanced Material Technologies, (Head: Prof. dr hab. Edward Chlebus), Krasinskiego 13a, 50 449 Wroclaw, Poland. Electronic address: patrycja.e.szymczyk@pwr.wroc.pl. 5. Department of Bioorganic Chemistry, Faculty of Chemistry, Wroclaw University of Technology, (Head: Dr hab. Piotr Mlynarz), C.K. Norwida 4/6, 50 373 Wroclaw, Poland. Electronic address: karolina.gluza@pwr.wroc.pl. 6. Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wroclaw, (Head: Dr hab. n. med. Marzenna Bartoszewicz), Borowska 211a 4, 50 556 Wroclaw, Poland. Electronic address: m.bartoszewicz@op.pl.
Abstract
BACKGROUND: Because of the suspicion that bisphosphonates enhance bacterial colonization, this study evaluated adhesion and biofilm formation by Streptococcus mutans 25175, Staphylococcus aureus 6538, and Pseudomonas aeruginosa 14454 reference strains on hydroxyapatite coated with clodronate, pamidronate, or zoledronate. MATERIAL AND METHODS: Bacterial strains were cultured on bisphosphonate-coated and noncoated hydroxyapatite discs. After incubation, nonadhered bacteria were removed by centrifugation. Biofilm formation was confirmed by scanning electron microscopy. Bacterial colonization was estimated using quantitative cultures compared by means with Kruskal-Wallis and post-hoc Student-Newman-Keuls tests. Modeling of the interactions between bisphosphonates and hydroxyapatite was performed using the Density Functional Theory method. RESULTS: Bacterial colonization of the hydroxyapatite discs was significantly higher for all tested strains in the presence of bisphosphonates vs. CONTROLS: Adherence in the presence of pamidronate was higher than with other bisphosphonates. Density Functional Theory analysis showed that the protonated amine group of pamidronate, which are not present in clodronate or zoledronate, forms two additional hydrogen bonds with hydroxyapatite. Moreover, the reactive cationic amino group of pamidronate may attract bacteria by direct electrostatic interaction. CONCLUSION: Increased bacterial adhesion and biofilm formation can promote osteomyelitis, cause failure of dental implants or bisphosphonate-coated joint prostheses, and complicate bone surgery in patients on bisphosphonates.
BACKGROUND: Because of the suspicion that bisphosphonates enhance bacterial colonization, this study evaluated adhesion and biofilm formation by Streptococcus mutans 25175, Staphylococcus aureus 6538, and Pseudomonas aeruginosa 14454 reference strains on hydroxyapatite coated with clodronate, pamidronate, or zoledronate. MATERIAL AND METHODS: Bacterial strains were cultured on bisphosphonate-coated and noncoated hydroxyapatite discs. After incubation, nonadhered bacteria were removed by centrifugation. Biofilm formation was confirmed by scanning electron microscopy. Bacterial colonization was estimated using quantitative cultures compared by means with Kruskal-Wallis and post-hoc Student-Newman-Keuls tests. Modeling of the interactions between bisphosphonates and hydroxyapatite was performed using the Density Functional Theory method. RESULTS: Bacterial colonization of the hydroxyapatite discs was significantly higher for all tested strains in the presence of bisphosphonates vs. CONTROLS: Adherence in the presence of pamidronate was higher than with other bisphosphonates. Density Functional Theory analysis showed that the protonated amine group of pamidronate, which are not present in clodronate or zoledronate, forms two additional hydrogen bonds with hydroxyapatite. Moreover, the reactive cationic amino group of pamidronate may attract bacteria by direct electrostatic interaction. CONCLUSION: Increased bacterial adhesion and biofilm formation can promote osteomyelitis, cause failure of dental implants or bisphosphonate-coated joint prostheses, and complicate bone surgery in patients on bisphosphonates.
Authors: Adam Junka; Anna Żywicka; Grzegorz Chodaczek; Mariusz Dziadas; Joanna Czajkowska; Anna Duda-Madej; Marzenna Bartoszewicz; Katarzyna Mikołajewicz; Grzegorz Krasowski; Patrycja Szymczyk; Karol Fijałkowski Journal: Sci Rep Date: 2019-02-04 Impact factor: 4.379
Authors: Sven Otto; Christoph Pautke; Daniel Arens; Philipp Poxleitner; Ursula Eberli; Dirk Nehrbass; Stephan Zeiter; Martin J Stoddart Journal: J Bone Miner Res Date: 2020-08-10 Impact factor: 6.741