| Literature DB >> 26026380 |
Treesukon Treebupachatsakul1, Koki Shioya1, Hikaru Nakazawa1, Takashi Kawaguchi2, Yasushi Morikawa1, Yosuke Shida1, Wataru Ogasawara3, Hirofumi Okada1.
Abstract
The capacity of Trichoderma reesei cellulase to degrade lignocellulosic biomass has been enhanced by the construction of a recombinant T. reesei strain expressing Aspergillus aculeatus β-glucosidase I. We have confirmed highly efficient ethanol production from converge-milled Japanese cedar by recombinant T. reesei expressing A. aculeatus β-glucosidase I (JN11). We investigated the ethanol productivity of JN11 and compared it with the cocktail enzyme T. reesei PC-3-7 with reinforced cellobiase activity by the commercial Novozyme 188. Results showed that the ethanol production efficiency under enzymatic hydrolysis of JN11 was comparable to the cocktail enzyme both on simultaneous saccharification and fermentation (SSF) or separate hydrolysis and fermentation (SHF) processes. Moreover, the cocktail enzyme required more protein loading for attaining similar levels of ethanol conversion as JN11. We propose that JN11 is an intrinsically economical enzyme that can eliminate the supplementation of BGL for PC-3-7, thereby reducing the cost of industrial ethanol production from lignocellulosic biomass.Entities:
Keywords: Aspergillus aculeatus BGL I; Ethanol; Separate hydrolysis and fermentation; Simultaneous saccharification and fermentation; Trichoderma reesei
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Year: 2015 PMID： 26026380 DOI： 10.1016/j.jbiosc.2015.04.015
Source DB: PubMed Journal: J Biosci Bioeng ISSN： 1347-4421 Impact factor: 2.894