Monika Litwin1, Joanna Dubis2, Katarzyna Arczyńska2, Aleksandra Piotrowska3, Anna Frydlewicz4, Maciej Karczewski5, Piotr Dzięgiel3, Wojciech Witkiewicz2. 1. Regional Specialist Hospital in Wroclaw, Research and Development Centre, Wroclaw, Poland litwin@wssk.wroc.pl. 2. Regional Specialist Hospital in Wroclaw, Research and Development Centre, Wroclaw, Poland. 3. Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland. 4. Department of Obstetrics and Gynaecology, Regional Specialist Hospital in Wroclaw, Research and Development Centre, Wroclaw, Poland. 5. Regional Specialist Hospital in Wroclaw, Research and Development Centre, Wroclaw, Poland Illimites Foundation, Wroclaw, Poland.
Abstract
BACKGROUND/AIM: Cancer stem cells (CSCs) constitute a sub-population of tumor cells that possess stem cell properties, such as self-renewal and the ability of differentiation. The presence of CSCs is associated with metastatic potential, treatment resistance and poor patient prognosis. Recently, aberrant expression of P-element induced wimpy testis proteins-PIWI (HIWI and HILI) has been identified in various types of tumors. The aim of the study was to evaluate the clinical significance of the HIWI and HILI expression and its relationship with cancer stem cells markers in 72 patients with colorectal carcinoma (CRC). MATERIALS AND METHODS: The expression level of HIWI and HILI and cancer stem cells markers in paired cancerous and non-cancerous tissues was measured by real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. Immunohistochemistry was performed to confirm the observed changes on mRNA level and detect tissue localization of PIWI proteins. RESULTS: Significantly higher mRNA levels of HIWI and decreased HILI mRNA were measured in colorectal cancer tissues compared to corresponding non-cancerous samples. The changes in HIWI mRNA level in cancer tissues were correlated with OCT4 expression. Positive correlations between HILI level and SOX2 were also observed in cancerous tissues. CONCLUSION: Our results indicate a reciprocal regulation between HIWI, HILI and some CSCs markers in colorectal cancer. Copyright
BACKGROUND/AIM: Cancer stem cells (CSCs) constitute a sub-population of tumor cells that possess stem cell properties, such as self-renewal and the ability of differentiation. The presence of CSCs is associated with metastatic potential, treatment resistance and poor patient prognosis. Recently, aberrant expression of P-element induced wimpy testis proteins-PIWI (HIWI and HILI) has been identified in various types of tumors. The aim of the study was to evaluate the clinical significance of the HIWI and HILI expression and its relationship with cancer stem cells markers in 72 patients with colorectal carcinoma (CRC). MATERIALS AND METHODS: The expression level of HIWI and HILI and cancer stem cells markers in paired cancerous and non-cancerous tissues was measured by real-time reverse transcription-polymerase chain reaction (RT-PCR) assay. Immunohistochemistry was performed to confirm the observed changes on mRNA level and detect tissue localization of PIWI proteins. RESULTS: Significantly higher mRNA levels of HIWI and decreased HILI mRNA were measured in colorectal cancer tissues compared to corresponding non-cancerous samples. The changes in HIWI mRNA level in cancer tissues were correlated with OCT4 expression. Positive correlations between HILI level and SOX2 were also observed in cancerous tissues. CONCLUSION: Our results indicate a reciprocal regulation between HIWI, HILI and some CSCs markers in colorectal cancer. Copyright
Authors: Monika Litwin; Anna Szczepańska-Buda; Aleksandra Piotrowska; Piotr Dzięgiel; Wojciech Witkiewicz Journal: Oncol Lett Date: 2017-03-28 Impact factor: 2.967
Authors: Markus Eckstein; Rudolf Jung; Katrin Weigelt; Danijel Sikic; Robert Stöhr; Carol Geppert; Abbas Agaimy; Verena Lieb; Arndt Hartmann; Bernd Wullich; Sven Wach; Helge Taubert Journal: Sci Rep Date: 2018-12-06 Impact factor: 4.379
Authors: Weiyao Li; Javier Martinez-Useros; Nuria Garcia-Carbonero; Maria J Fernandez-Aceñero; Luis Ortega-Medina; Sandra Garcia-Botella; Elia Perez-Aguirre; Luis Diez-Valladares; Jesus Garcia-Foncillas Journal: J Clin Med Date: 2019-08-22 Impact factor: 4.241
Authors: Ewa E Hennig; Anna Kluska; Magdalena Piątkowska; Maria Kulecka; Aneta Bałabas; Natalia Zeber-Lubecka; Krzysztof Goryca; Filip Ambrożkiewicz; Jakub Karczmarski; Tomasz Olesiński; Łukasz Zyskowski; Jerzy Ostrowski Journal: Biology (Basel) Date: 2021-05-25