Kelly A Hug1, Lauren Anthony2, Dalia Eldeiry3, Jenna Benson2, Elyse Wheeler2, Shaker Mousa4, Binshan Shi5. 1. Department of Health Sciences, Albany College of Pharmacy and Health Sciences, Albany, NY, U.S.A. Department of Cytology, Samaritan Hospital, Troy, NY, U.S.A. 2. Department of Health Sciences, Albany College of Pharmacy and Health Sciences, Albany, NY, U.S.A. 3. Department of Cytology, Samaritan Hospital, Troy, NY, U.S.A. 4. The Pharmaceutical Research Institute, Albany College of Pharmacy and Health Sciences, Albany, NY, U.S.A. 5. Department of Health Sciences, Albany College of Pharmacy and Health Sciences, Albany, NY, U.S.A. Binshan.Shi@acphs.edu.
Abstract
BACKGROUND/AIM: miR-21 is a common OncomiR in human cancer. The present study analyzed the distribution and expression of miR-21 in breast tumor tissues so as to examine the role of miR-21 in the carcinogenesis of breast cancer. MATERIALS AND METHODS: Sixteen malignant and 10 benign breast tissue specimens were analyzed using a miRNA chromogenic in situ hybridization (CISH) assay. The locations of miR-21 CISH-positive cells in breast tissues were observed and its expression level was semi-quantified by ISH scoring. RESULTS: Positive in situ staining of miR-21 was detected in the cytoplasm of malignant epithelial cells in most of the high-grade infiltrating ductal carcinoma specimens. miR-21-positive spindle-like cells were found to surround tumor cell islands. High miR-21 ISH scores were correlated with positive lymph node status. miR-21 expression was low in most types of benign breast tissues. CONCLUSION: miR-21 is a potential biomarker for breast cancer prognosis. Copyright
BACKGROUND/AIM: miR-21 is a common OncomiR in humancancer. The present study analyzed the distribution and expression of miR-21 in breast tumor tissues so as to examine the role of miR-21 in the carcinogenesis of breast cancer. MATERIALS AND METHODS: Sixteen malignant and 10 benign breast tissue specimens were analyzed using a miRNA chromogenic in situ hybridization (CISH) assay. The locations of miR-21 CISH-positive cells in breast tissues were observed and its expression level was semi-quantified by ISH scoring. RESULTS: Positive in situ staining of miR-21 was detected in the cytoplasm of malignant epithelial cells in most of the high-grade infiltrating ductal carcinoma specimens. miR-21-positive spindle-like cells were found to surround tumor cell islands. High miR-21 ISH scores were correlated with positive lymph node status. miR-21 expression was low in most types of benign breast tissues. CONCLUSION:miR-21 is a potential biomarker for breast cancer prognosis. Copyright
Authors: Muhammad Noor Diansyah; Ami Ashariati Prayogo; Made Putra Sedana; Merlyna Savitri; Pradana Zaky Romadhon; Putu Niken Ayu Amrita; Andi Yasmin Wijaya; Winona May Hendrata; Ugroseno Yudho Bintoro Journal: Turk J Med Sci Date: 2021-04-30 Impact factor: 0.973