| Literature DB >> 26024626 |
Gabriele Rink1, Erwin A Scharberg, Peter Bugert.
Abstract
PCR with sequence-specific primers (PCR-SSP) is a cost-effective and robust method for the analysis of single nucleotide polymorphisms (SNPs). Many blood group antigens and the antithetic antigens are based on a diallelic SNP in the coding region of the corresponding blood group gene. Here, we describe PCR-SSP protocols for genotyping 24 blood group antigens based on 12 diallelic SNPs. We also provide protocols for molecular determination of the rare blood group phenotypes Yk(a-) and Vel-.Mesh：
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Year: 2015 PMID： 26024626 DOI： 10.1007/978-1-4939-2690-9_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745