| Literature DB >> 26024136 |
Abstract
The whole nucleotide sequence of the chloroplast genome from Capsella rubella is determined in this study using short Illumina sequence data from public database. The circular double-stranded DNA, which consists of 154,601 base pairs (bp) in size, contains a pair of inverted repeats (IRa and IRb) of 26,462 bp each, which are separated by a small and large single-copies (SSC and LSC) of 17,855 and 83,822 bp, respectively. The overall GC content of the chloroplast genome is 36.54% and the GC contents of LSC, IRs and SSC are 34.33%, 42.38% and 29.61% separately. One hundred and twelve unique genes were annotated, including 78 protein-coding genes, 30 tRNA genes and 4 rRNA genes. Among these, 16 are duplicated in the inverted repeat regions, 15 genes contained 1 intron, and 3 genes (rps12, clpP and ycf3) comprised of 2 introns. Two protein genes (rps19 and ycf1) span in the boundaries of LSC-IR and IR-SSC to produce two partial pseudogenes.Entities:
Keywords: CLC workbench; Capsella rubella; chloroplast genome; next-generation sequencing
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Year: 2015 PMID: 26024136 DOI: 10.3109/19401736.2015.1038804
Source DB: PubMed Journal: Mitochondrial DNA A DNA Mapp Seq Anal ISSN: 2470-1394 Impact factor: 1.514