| Literature DB >> 26024014 |
Přemysl Pejchar1, Jan Martinec.
Abstract
The first indication of the aluminum (Al) toxicity in plants growing in acidic soils is the cessation of root growth, but the detailed mechanism of Al effect is unknown. Here we examined the impact of Al stress on the activity of non-specific phospholipase C (NPC) in the connection with the processes related to the plasma membrane using fluorescently labeled phosphatidylcholine. We observed a rapid and significant decrease of labeled diacylglycerol (DAG), product of NPC activity, in Arabidopsis seedlings treated with AlCl₃. Interestingly, an application of the membrane fluidizer, benzyl alcohol, restored the level of DAG during Al treatment. Our observations suggest that the activity of NPC is affected by Al-induced changes in plasma membrane physical properties.Entities:
Keywords: Arabidopsis thaliana; BA, benzyl alcohol; BODIPY; BODIPY, 4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene; BY-2, Bright Yellow 2; DAG, diacylglycerol; HP-TLC, high-performance thin-layer chromatography; MS, Murashige-Skoog; NPC, non-specific phospholipase C; PA, phosphatidic acid; PC, phosphatidylcholine; PC-PLC, phosphatidylcholine-specific phospholipase C; PI-PLC, phosphatidylinositol-specific phospholipase C; PIP2, phosphatidylinositol 4, 5-bisphosphate; PLD, phospholipase D; PM, plasma membrane.; aluminum toxicity; benzyl alcohol; diacylglycerol; membrane fluidity; non-specific phospholipase C
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Year: 2015 PMID： 26024014 PMCID： PMC4622580 DOI： 10.1080/15592324.2015.1031938
Source DB: PubMed Journal: Plant Signal Behav ISSN： 1559-2316