S Sabiha Shaheen1, Padma Reddy2, Srikanth Reddy2, Dolar Doshi2, Suhas Kulkarni3, Manoj Kumar4. 1. Postgraduate Student, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences & Hospital , Hyderabad, India . 2. Reader, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences & Hospital , Hyderabad, India . 3. Professor & Head, Department of Public Health Dentistry, Panineeya Institute of Dental Sciences & Hospital , Hyderabad, India . 4. Scientist, Department of Microbiology and Immunology, National Institute of Nutrition, ICMR , Hyderabad, India .
Abstract
AIM: To determine and compare the antimicrobial efficacy of ten commercially available herbal dentifrices against specific strains of oral microflora using a standard diffusion method at full strength and 1:1 dilution at 24 h. MATERIALS AND METHODS: The standard strains of Streptococcus. mutans (ATCC 21293), Streptococcus sangius (MTCC 442), Actinomyces viscosus (ATCC 3268), Staphylococcus aureus (ATCC 2592), Streptococcus pyogenes (MTCC 442) and Candida albicans (ATCC 183) were obtained. Antimicrobial efficacy of the dentifrices was tested in triplicate, at full strength and 1:1 dilution with the sterile water using a standard diffusion method for 24 h at 37°C. The antimicrobial efficacy was tested by observing the zones of inhibition in millimeters surrounding disk containing the dentifrice. Mean standard deviation and standard error of mean of the inhibitory zones was calculated for each herbal dentifrice. p<0.05 was considered statistically significant. RESULTS: Danth Kanthi (DK) was the most effective against all the microorganisms producing larger zones of inhibition at 24 h (F.S - 40±1.5; 1:1 dilution - 40±2.71). Amar Premium (AP) also produced larger zones of inhibition against all microorganisms except S. aureus. Of all the dentifrices, least zones of inhibitions i.e., around 5 mm was observed against S. aureus by Amar Premium (AP) and Dabur Babool (DB) at 24 h. CONCLUSION: Based on the results of the present study, it can be concluded that all herbal dentifrices exhibited antimicrobial activity against the selected oral microorganisms, with DK being the most effective. Hence, it can be inferred that herbal dentifrices can also be recommended like the conventional formulations.
AIM: To determine and compare the antimicrobial efficacy of ten commercially available herbal dentifrices against specific strains of oral microflora using a standard diffusion method at full strength and 1:1 dilution at 24 h. MATERIALS AND METHODS: The standard strains of Streptococcus. mutans (ATCC 21293), Streptococcus sangius (MTCC 442), Actinomyces viscosus (ATCC 3268), Staphylococcus aureus (ATCC 2592), Streptococcus pyogenes (MTCC 442) and Candida albicans (ATCC 183) were obtained. Antimicrobial efficacy of the dentifrices was tested in triplicate, at full strength and 1:1 dilution with the sterile water using a standard diffusion method for 24 h at 37°C. The antimicrobial efficacy was tested by observing the zones of inhibition in millimeters surrounding disk containing the dentifrice. Mean standard deviation and standard error of mean of the inhibitory zones was calculated for each herbal dentifrice. p<0.05 was considered statistically significant. RESULTS: Danth Kanthi (DK) was the most effective against all the microorganisms producing larger zones of inhibition at 24 h (F.S - 40±1.5; 1:1 dilution - 40±2.71). Amar Premium (AP) also produced larger zones of inhibition against all microorganisms except S. aureus. Of all the dentifrices, least zones of inhibitions i.e., around 5 mm was observed against S. aureus by Amar Premium (AP) and Dabur Babool (DB) at 24 h. CONCLUSION: Based on the results of the present study, it can be concluded that all herbal dentifrices exhibited antimicrobial activity against the selected oral microorganisms, with DK being the most effective. Hence, it can be inferred that herbal dentifrices can also be recommended like the conventional formulations.
Authors: Martinus J Verkaik; Henk J Busscher; Debbie Jager; Anje M Slomp; Frank Abbas; Henny C van der Mei Journal: J Dent Date: 2010-12-30 Impact factor: 4.379
Authors: M A Botelho; N A P Nogueira; G M Bastos; S G C Fonseca; T L G Lemos; F J A Matos; D Montenegro; J Heukelbach; V S Rao; G A C Brito Journal: Braz J Med Biol Res Date: 2007-03 Impact factor: 2.590