Literature DB >> 26023287

DNA Barcoding of the parasitoid wasp subfamily Doryctinae (Hymenoptera: Braconidae) from Chamela, Mexico.

Daniela Gutiérrez-Arellano1, Claudia Renata Gutiérrez-Arellano2, Alejandro Zaldívar-Riverón1.   

Abstract

Background and aims. The Doryctinae is a considerably diverse, poorly studied group of parasitoid wasps and one of the most diverse subfamilies within Braconidae. Taxonomic knowledge of this group remains highly incomplete, specially in the tropics. In Mexico, it has been reported as the subfamily with the highest number of recorded genera. A preliminary Barcoding study carried out in the Chamela region, located near the Mexican pacific coast in Jalisco, identified 185 barcoding species of Dorytinae assigned to 19 identified doryctine genera. This work updates the later study, representing a three years effort to assess the species richness of this subfamily for the Chamela region. Materials and methods. Ten collecting field trips of 5 to 10 days each were carried out from June 2009 to May 2011. A 2% divergence criterion using the BIN system implemented in BOLD was followed in order to establish species boundaries among the specimens that were collected. Results and conclusions. A total of 961 specimens were collected, from which 883 COI sequences were obtained. The sequences generated corresponded to 289 barcoding species and 30 identified genera. The most speciose genera were Heterospilus Haliday (170 spp.), Ecphylus Förster (19 spp.), Allorhogas Gahan (15 spp.) and Callihormius Ashmead (14 spp.). Addition of previously collected material increased the diversity of the subfamily in the region to 34 genera and 290 species. Paraphyly of Heterospilus with respect to Neoheterospilus and Heterospathius was again recovered. Twenty new species and two new genera (Sabinita Belokobylskij, Zaldívar-Riverón et Martínez, Ficobolus Martínez, Belokobylskij et Zaldívar-Riverón) have been described so far from the material collected in this work.

Entities:  

Keywords:  COI; Ichneumonoidea; Neotropics; faunistic study; species richness

Year:  2015        PMID： 26023287      PMCID： PMC4442253          DOI： 10.3897/BDJ.3.e5109

Source DB:  PubMed          Journal:  Biodivers Data J        ISSN： 1314-2828


Introduction

Biodiversity inventories represent an integral component for the adequate management of natural resources of any country. In the case of faunistic studies, however, these rarely include arthropod taxa due to their considerably high diversity and scarce taxonomic knowledge. In this context, DNA Barcoding (Hebert et al. 2003a) represents a fast, valuable approach to built species inventories of highly diverse, poorly known invertebrate groups. With over 1,300 described and almost 3,000 estimated species, is one the four most diverse subfamilies of braconid parasitoid wasps together with the , and (Jones et al. 2009, Yu et al. 2012). Doryctine wasps are mainly known to be idiobiont ectoparasitoids of xylophagous and bark boring coleopteran larvae, though other host groups (e.g. , , ) and biologies (e.g. phytophagy, endoparasitoidism) have also been reported (Belokobylskij 1992, Belokobylskij et al. 2004). Several doryctine species are known to be parasitoids of insect pests (LaSalle and Gauld 1993), therefore they play an important role for maintaining the balance of terrestrial ecosystems (Hawkins and Hochberg 1994). The taxonomic knowledge of the is still far from complete, especially in the tropics and subtropics, where most of its species richness is known to occur (Belokobylskij 1992). In the Mexican territory, this was recently reported as the subfamily with the highest number of recorded genera (63 genera; Coronado-Blanco and Zaldívar-Riverón 2014). However, this number contrasts with its scarce number of described species that have been reported for the country. Recently, Zaldívar-Riverón et al. (2010) showed the preliminary results of a DNA Barcoding survey of the from Chamela Biological Station (CBS) in Mexico, which located near the Pacific coast in the state of Jalisco and is mainly composed of tropical dry forest (Noguera et al. 2002). Zaldívar-Riverón et al. (2010)​ not only revealed and extraordinary, mostly undescribed species richness for this group (185 Barcoding and 20 recognized genera), but also showed some previously undetected taxonimic problems. Here we show the final list of the DNA Barcoding species inventory of the from the CBS, which was carried out during three years of collecting effort. We updated the list of genera that occur in the region and show their number of DNA Barcoding species based on the barcoding index criterion (BIN; Ratnasingham and Hebert 2013). This faunistic study highlights the extraordinary, neglected species richness of this parasitoid group in the Mexican territory, and is also serving as a basis for the subsequent description of several new taxa.

Materials and methods

Study area

Specimens belonging to the subfamily were collected in the Chamela biological station (Fig. 1), owned by the Instituto de Biología, Universidad Nacional Autónoma de México (UNAM). This station is located within the Chamela–Cuixmala Biosphere Reserve (CCBR), near the Pacific coast in the state of Jalisco(19°29' N, 105° 02' W). The prevailing climate in the area is tropical sub-humid (mean annual temperature 24.6 °C, annual precipitation 788 mm; García 1988). There is a strong seasonality in the region, with alternating rainy (Jul- Oct) and dry (Nov-Jun) seasons. Altitude ranges from 0 to 500 meters above sea level. The dominant type of vegetation is tropical dry forest, which is characteristic of the Mexican Pacífic coast, though patches of tropical evergreen forest, coastal dune, xeric shrubland and mangrove are also present (Rzedowski 1978, Noguera et al. 2002). The relevance of this area lies in the variety of habitats it supports, as well as in its high endemic component of plants, vertebrates and insects (Noguera et al. 2002).
Figure 1.

Study area. The Chamela Biological Station (IB-UNAM), located within the Chamela-Cuixmala Biosphere Reserve in the estate of Jalisco, Mexico.

Specimen sampling

A total of 10 collecting trips of 5 to 10 days each were carried out from June 2009 to May 2011. Collects were performed both during the rainy and dry seasons in order to have a complete representation of the examined group through the year. Specimens were collected in 24 sites within the CBS boundaries (Table 1), using for this: 1) malaise traps, 2) light traps, 3) yellow pan traps, as well as 5) sweep nets for at least 4 h per day (Marshall 1994). The collected materials were preserved in 96% ethanol and kept at -20 °C. All specimens were sorted out at subfamily level following the taxonomic key of New World genera of (Wharton et al. 1997). Doryctine specimens were then identified to genus level using the relevant literature (Marsh 1997, Marsh 2002) and subsequent descriptions of genera (e.g. Zaldívar-Riverón et al. 2014).
Table 1.

Sampling sites and collecting techniques employed in this study.

SITELATITUDELONGITUDEALTITUDETRAP TYPE
Near CBS 19.4985 -105.0441 92Yellow pan trap
Camino Zarco/Chachalaca 19.4956 -105.0393 30Sweep net
Camino Búho 19.4990 -105.0412 74Malaise
19.4988 -105.0404 65Light trap
Camino Chachalaca 19.4993 -105.0383 56Sweep net, Malise trap, Yellow pan trap
19.4978 -105.0445 120Sweep net
Camino Calandria 19.5084 -105.0378 120Yellow pan trap, sweep net, light trap, Malaise trap
Eje central/Camino Calandria 19.5002 -105.0425 49Yellow pan
19.5039 -105.0335 52Sweep net
Camino Calandria/Camino Chachalaca 19.5049 -105.0377 52Sweep net
Fundación-Cuixmala/Poza del Jaguar 19.4292 -104.9796 66Sweep net
Fundación-Cuixmala/El Sendero 19.4192 -104.9732 61Sweep net, Malaise trap
Behind CBS dinning room 19.4978 -105.0445 120Sweep net
Camino Ardilla 19.5042 -105.0379 39Sweep net
Eje central 19.5085 -105.0366 32Sweep net
Calandria(Arroyo Calandria) 19.5036 -105.0364 62Sweep net
19.5002 -105.0353 109Sweep net
Camino Búho/ Chachalaca 19.4991 -105.0421 68"O" Trap
19.4978 -105.0444 106Sweep net
Camino Antiguo 19.5047 -105.0367 59Sweep net

DNA sequencing

DNA samples were obtained from a single hind leg and sent for DNA extraction and amplification to the Canadian Center for DNA Barcoding at University of Guelph, Ontario (see detailed laboratory protocols in Smith et al. 2008). A 615–658 bp fragment corresponding to the standard animal DNA barcoding locus (cytochrome c oxidase subunit I mtDNA gene, COI) was amplified for the collected samples using both LepF1/LepRI (Hebert et al. 2003b) and LCO1490/HCO2198 (Folmer et al. 1994) primers. Sequences were edited with Sequencher 4.0.5 (Gene Codes Corp.) and aligned manually based on their translated amino acids. All of the COI sequences generated are deposited in GenBank (accession numbers GU715182-288, HM420734-5, HM434309-544, HM882254, HQ200960-201008, HQ201239-54). All sequences and their specimen information are available in the project file “Parasitoid Wasps (: ) of Chamela–Cuixmala Biosphere Reserve” (ASDORproject) in the projects section of the Barcode of Life Data Systems (www.barcodinglife.com)

Species boundaries

Species boundaries were established following a 2% divergence criterion (Hebert et al. 2003a). This criterion has been shown to represent a fast, generally reliable tool for exploring species richness in different animal taxa (Ratnasingham and Hebert 2013). This established genetic distance is based on the assumption that COI divergences usually do not exceed a 2% divergence within a recognized species, whereas different species generally show a higher divergence (Hebert et al. 2003a). Sequences divergence of the sequenced specimens were obtained using the K2P distance model (Kimura 1980). A neighbourjoining (NJ) tree and an accumulation curve were reconstructed as implemented in the BOLD system (Barcode of Life Data System, www.boldsystems.org). BINs were obtained for each specimen from the BOLD system, and this was employed to establish the number of barcoding species.

Data resources

ASDOR Project link Search as: ASDOR http://www.boldsystems.org/index.php/Public_BINSearch?searchtype=records GenBank link Search as: Nucleotide + accession number http://www.ncbi.nlm.nih.gov/genbank/

Checklists

Checklist of described doryctine species from the Chamela Biological Station

Martínez and Zaldívar-Riverón 2013 http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Type status: Paratype. Occurrence: catalogNumber: ASDOR472-10; recordedBy: Zaldívar, Zaragoza, Ibarra; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-20-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR517-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.5; decimalLongitude: -105.039; Event: eventDate: 02-21-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR518-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.5; decimalLongitude: -105.039; Event: eventDate: 02-21-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR519-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.5; decimalLongitude: -105.039; Event: eventDate: 02-21-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR764-10; recordedBy: Zaldívar, Salinas, Ramos; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.035; Event: eventDate: 03-28-10 Type status: Holotype. Occurrence: catalogNumber: CNIN777; recordedBy: Zaldívar, Zaragoza, Ibarra; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: coccolobae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11

Distribution

Chamela, Jalisco, Mexico

Notes

n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013) Martínez and Zaldívar-Riverón 2014 http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Type status: Paratype. Occurrence: catalogNumber: ASDOR042-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: crassifemur; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Holotype. Occurrence: catalogNumber: ASDOR043-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: crassifemur; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-23-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013) Martínez and Zaldívar-Riverón 2013 http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Type status: Paratype. Occurrence: catalogNumber: ASDOR081-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 09-24-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR350-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 09-03-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR456-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.038; Event: eventDate: 11-20-09 Type status: Holotype. Occurrence: catalogNumber: ASDOR457-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.038; Event: eventDate: 11-20-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013) Martínez and Zaldívar-Riverón 2013 http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Type status: Paratype. Occurrence: catalogNumber: ASDOR758-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR759-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR760-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR762-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.496; decimalLongitude: -105.039; Event: eventDate: 03-28-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR763-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.496; decimalLongitude: -105.039; Event: eventDate: 03-28-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR776-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10 Type status: Holotype. Occurrence: catalogNumber: ASDOR777-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: marshi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013) Martínez and Zaldívar-Riverón 2013 http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Type status: Paratype. Occurrence: catalogNumber: ASDOR458-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: parvus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 11-21-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR470-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: parvus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-20-10 Type status: Holotype. Occurrence: catalogNumber: ASDOR778-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: parvus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013) Martínez and Zaldívar-Riverón 2013 http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870-34532013000100008 Type status: Paratype. Occurrence: catalogNumber: ASDOR321-10; recordedBy: Clebsch, Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.429; decimalLongitude: -104.98; Event: eventDate: 09-05-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR488-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-20-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR607-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 02-23-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR745-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.496; decimalLongitude: -105.039; Event: eventDate: 03-28-10 Type status: Holotype. Occurrence: catalogNumber: ASDOR767-10; recordedBy: Zaldívar, Salinas, Ramos; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.035; Event: eventDate: 03-28-10 Type status: Paratype. Occurrence: catalogNumber: ASDOR768-10; recordedBy: Zaldívar, Salinas, Ramos; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: scotti; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.035; Event: eventDate: 03-28-10 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2013) Zaldivar-Riverón and Belokobylskij 2014 http://onlinelibrary.wiley.com/doi/10.1111/syen.12078/abstract;jsessionid=0441E95BA6DBCB93BF1257DF4E1A3B0D.f03t03?deniedAccessCustomisedMessage=&userIsAuthenticated=false Type status: Paratype. Occurrence: catalogNumber: ASDOR446-10; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-25-09 Type status: Holotype. Occurrence: catalogNumber: ASDOR447-10; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-25-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Zaldívar-Riverón et al. 2014) Martínez, Zaldívar-Riverón, Ceccarelli and Shaw 2012 http://zookeys.pensoft.net/articles.php?id=2399 Type status: Paratype. Occurrence: catalogNumber: ASDOR075-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: ecmahla; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Holotype. Occurrence: catalogNumber: ASDOR076-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: ecmahla; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012) Martínez, Zaldívar-Riverón, Ceccarelli and Shaw 2013 http://zookeys.pensoft.net/articles.php?id=2400 Type status: Paratype. Occurrence: catalogNumber: ASDOR551-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: macrophthalma; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 02-02-11 Type status: Paratype. Occurrence: catalogNumber: ASDOR555-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: macrophthalma; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 02-02-11 Type status: Paratype. Occurrence: catalogNumber: ASDOR761-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: macrophthalma; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042; Event: eventDate: 02-25-10 Type status: Holotype. Occurrence: catalogNumber: CNIN795; recordedBy: Zaldívar, Zaragoza, Ibarra; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: macrophthalma; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012) Marsh 2002 http://ejournal.narotama.ac.id/files/The%20genus%20Iare%20Barbalho%20and%20Penteado-Dias%20(Hymenoptera%20Braconidae%20Doryctinae)%20in%20Mexico,%20with%20the%20description%20of%20two%20new%20species.pdf Type status: Other material. Occurrence: catalogNumber: ASDOR022-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Other material. Occurrence: catalogNumber: ASDOR023-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Other material. Occurrence: catalogNumber: ASDOR025-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Other material. Occurrence: catalogNumber: ASDOR026-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Other material. Occurrence: catalogNumber: ASDOR029-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Other material. Occurrence: catalogNumber: ASDOR031-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Other material. Occurrence: catalogNumber: ASDOR035-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: belokobylskiji; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Mexico and Costa Rica Martínez, Zaldívar-Riverón, Ceccarelli and Shaw 2010 http://ejournal.narotama.ac.id/files/The%20genus%20Iare%20Barbalho%20and%20Penteado-Dias%20(Hymenoptera%20Braconidae%20Doryctinae)%20in%20Mexico,%20with%20the%20description%20of%20two%20new%20species.pdf Type status: Paratype. Occurrence: catalogNumber: ASDOR020-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR021-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR027-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Holotype. Occurrence: catalogNumber: ASDOR028-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR096-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: cheguevarai; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Martínez et al. 2010) Martínez, Zaldívar-Riverón, Ceccarelli and Shaw 2011 http://ejournal.narotama.ac.id/files/The%20genus%20Iare%20Barbalho%20and%20Penteado-Dias%20(Hymenoptera%20Braconidae%20Doryctinae)%20in%20Mexico,%20with%20the%20description%20of%20two%20new%20species.pdf Type status: Paratype. Occurrence: catalogNumber: ASDOR030-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: mexicanus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Holotype. Occurrence: catalogNumber: ASDOR033-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: mexicanus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Martínez et al. 2010) Zaldívar-Riverón, Martínez, Ceccarelli and Shaw 2012 http://zookeys.pensoft.net/articles.php?id=2399 Type status: Paratype. Occurrence: catalogNumber: CNIN741; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Holotype. Occurrence: catalogNumber: CNIN798; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-27-09 Type status: Paratype. Occurrence: catalogNumber: CNIN798; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Paratype. Occurrence: catalogNumber: CNIN799; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Type status: Paratype. Occurrence: catalogNumber: CNIN800; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: jaliscoensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012) Zaldívar-Riverón, Martínez, ceccarelli and Shaw 2013 http://zookeys.pensoft.net/articles.php?id=2399 Type status: Paratype. Occurrence: catalogNumber: CNIN739; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: pacificus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11 Type status: Holotype. Occurrence: catalogNumber: CNIN740; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: pacificus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 06-26-09 Type status: Paratype. Occurrence: catalogNumber: CNIN742; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: pacificus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11 Type status: Paratype. Occurrence: catalogNumber: CNIN743; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: pacificus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044; Event: eventDate: 05-05-11 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012) Belokobylskij, Zaldivar-Riverón, Coronado-Blanco 2014 http://dx.doi.org/10.11646/zootaxa.3795.4.2 Type status: Holotype. Occurrence: recordedBy: Toledo; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: fridae; Location: country: Mexico; stateProvince: Morelos; municipality: Tepalcingo; locality: El Limón; decimalLatitude: 12.52; decimalLongitude: -98.94; Event: eventDate: 10-13-12 Type status: Paratype. Occurrence: recordedBy: Toledo, Hinterholzer, Martínez; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: fridae; Location: country: Mexico; stateProvince: Puebla; municipality: Jolalpan; locality: Rancho el Salado; decimalLatitude: 18.33; decimalLongitude: -98.98; Event: eventDate: 10-07-10 Type status: Paratype. Occurrence: recordedBy: Toledo; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: fridae; Location: country: Mexico; stateProvince: Morelos; municipality: Tlaquiltenango; locality: Santiopa; decimalLatitude: 18.44; decimalLongitude: -98.95; Event: eventDate: 07-06-13 Type status: Paratype. Occurrence: recordedBy: Toledo; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: fridae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.49; decimalLongitude: -105.044; Event: eventDate: 06-06-11 Central Mexico and Jalisco n.sp. described from specimens collected in this study (Belokobyskij et al. 2014) Marínez and Zaldívar-Riverón 2010 http://www.conabio.gob.mx/institucion/proyectos/resultados/HB033_Neoheterospilus%202010.pdf Type status: Holotype. Occurrence: catalogNumber: ASDOR053-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044 Type status: Paratype. Occurrence: catalogNumber: ASDOR054-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044 Type status: Paratype. Occurrence: catalogNumber: ASDOR095-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038 Type status: Other material. Occurrence: catalogNumber: ASDOR108-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038 Type status: Other material. Occurrence: catalogNumber: ASDOR148-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.497; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR202-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR216-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.045 Type status: Paratype. Occurrence: catalogNumber: ASDOR343-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.429; decimalLongitude: -104.98 Type status: Paratype. Occurrence: catalogNumber: ASDOR392-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044 Type status: Paratype. Occurrence: catalogNumber: ASDOR418-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR473-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042 Type status: Paratype. Occurrence: catalogNumber: ASDOR482-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042 Type status: Paratype. Occurrence: catalogNumber: ASDOR492-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042 Type status: Paratype. Occurrence: catalogNumber: ASDOR549-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.509; decimalLongitude: -105.037 Type status: Paratype. Occurrence: catalogNumber: ASDOR550-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.509; decimalLongitude: -105.037 Type status: Paratype. Occurrence: catalogNumber: ASDOR565-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044 Type status: Paratype. Occurrence: catalogNumber: ASDOR566-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044 Type status: Paratype. Occurrence: catalogNumber: ASDOR567-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044 Type status: Paratype. Occurrence: catalogNumber: ASDOR601-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR625-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR627-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR632-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR633-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR697-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042 Type status: Other material. Occurrence: catalogNumber: ASDOR718-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.035 Type status: Other material. Occurrence: catalogNumber: ASDOR737-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.496; decimalLongitude: -105.039 Type status: Other material. Occurrence: catalogNumber: ASDOR769-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042 Type status: Other material. Occurrence: catalogNumber: ASDOR770-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.042 Type status: Other material. Occurrence: catalogNumber: ASDOR772-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.5; decimalLongitude: -105.039 Type status: Other material. Occurrence: catalogNumber: ASDOR773-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038 Type status: Other material. Occurrence: catalogNumber: ASDOR775-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038 Type status: Other material. Occurrence: catalogNumber: ASDOR789-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044 Type status: Other material. Occurrence: catalogNumber: ASDOR816-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044 Type status: Other material. Occurrence: catalogNumber: ASDOR820-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044 Type status: Other material. Occurrence: catalogNumber: ASDOR822-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.49; decimalLongitude: -105.038 Type status: Other material. Occurrence: catalogNumber: ASDOR853-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.044 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Martínez and Zaldívar-Riverón 2010) Reséndiz-Flores, Nunes and Zaldívar Riverón 2014 http://www.ib.unam.mx/m/revista/pdfs/05.-_1581_1.pdf Type status: Holotype. Occurrence: catalogNumber: ASDOR016-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: crypticus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-25-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR017-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: crypticus; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-26-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Reséndiz-Flores et al. 2014) Reséndiz-Flores, Nunes and Zaldívar Riverón 2014 http://www.ib.unam.mx/m/revista/pdfs/05.-_1581_1.pdf Type status: Paratype. Occurrence: catalogNumber: ASDOR018-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-26-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR019-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.499; decimalLongitude: -105.038; Event: eventDate: 06-27-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR355-10; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 09-04-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR357-10; recordedBy: Clebsch, Zaldívar, Polaszek; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 09-03-09 Type status: Holotype. Occurrence: catalogNumber: ASDOR463-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: mariachi; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.504; decimalLongitude: -105.038; Event: eventDate: 11-20-09 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Reséndiz-Flores et al. 2014) Zaldívar-Riverón, Martínez, ceccarelli and Shaw 2014 http://zookeys.pensoft.net/articles.php?id=2399 Type status: Holotype. Occurrence: catalogNumber: ASDOR464-10; recordedBy: Clebsch, Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: cuixmalensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.419; decimalLongitude: -104.973; Event: eventDate: 09-03-09 Type status: Paratype. Occurrence: catalogNumber: ASDOR514-10; recordedBy: Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: cuixmalensis; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038; Event: eventDate: 02-20-10 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Zaldivar-Riveron et al. 2012) Belokobylskij, Zaldivar-Riverón and Martínez 2014 http://onlinelibrary.wiley.com/doi/10.1111/syen.12078/abstract;jsessionid=0441E95BA6DBCB93BF1257DF4E1A3B0D.f03t03?deniedAccessCustomisedMessage=&userIsAuthenticated=false Type status: Holotype. Occurrence: catalogNumber: ASDOR082-09; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: mexicana; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.497; decimalLongitude: -105.038; Event: eventDate: 07-02-12 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Zaldívar-Riverón et al. 2014) Belokobylskij and Zaldivar-Riverón 2014 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4137313/pdf/zookeys-427-059.pdf Type status: Paratype. Occurrence: catalogNumber: ASDOR371-10; recordedBy: Clebsch, Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR372-10; recordedBy: Clebsch, Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR373-10; recordedBy: Clebsch, Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR375-10; recordedBy: Clebsch, Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Paratype. Occurrence: catalogNumber: ASDOR432-10; recordedBy: Clebsch, Zaldívar; sex: male; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Type status: Holotype. Occurrence: catalogNumber: ASDOR433-10; recordedBy: Zaldívar; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: chamelae; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.505; decimalLongitude: -105.038 Chamela, Jalisco, Mexico n.sp. described from specimens collected in this study (Belokobylskij and Zaldivar-Riveron 2014) Marsh 1993 http://doryctinaekey.myspecies.info/file/339 Type status: Other material. Occurrence: catalogNumber: ASDOR001-09; recordedBy: Clebsch, Zaldívar, Polaszek; sex: female; Taxon: kingdom: ; phylum: ; class: ; order: ; family: ; genus: ; specificEpithet: variegata; Location: country: Mexico; stateProvince: Jalisco; municipality: La Huerta; locality: Chamela Biostation; decimalLatitude: 19.498; decimalLongitude: -105.044; Event: eventDate: 06-24-09 Mexico, Guatemala, Panama and Costa Rica

Analysis

A total of 961 specimens were collected during the ten field trips, of which 883 COI sequences were generated. Fifteen of these sequences had a length lower than 500 bp and thus did not receive a BIN, though they were included in the analysis. The sequences generated belonged to 289 barcoding species and 30 identified genera, though four barcoding species could not be assigned to any genus (Table 2). Haliday was the most speciose genera with 170 barcoding species, followed by Förster (19 spp.), Gahan (15 spp.) and Ashmead (14 spp.). Two species belonging to two additional genera ( Roman; , Belokobyskij et al. 2014 were foundfound in the entomological collection at the CBS, increasing the diversity of doryctine genera and species present in the region to 33 and 289, respectively.
Table 2.

Doryctine genera and number of barcoding species identified in this study (Total 289).

* Species identified only based on morphological evidence.

GeneraBarcoding species
Allorhogas Gahan15
Caenophanes Förster1
Callihormius Ashmead14
Coiba Marsh7
Concurtisella Roman1
Curtisella Spinola4
Doryctinus* Roman1
Ecphylus Förster19
Ficobolus Martínez, Belokobylskij et Zaldívar-Riverón1
Glyptocolastes Ashmead8
Heerz Marsh2
Heterospathius Barbalho et Penteado-Dias1
Heterospilus Haliday170
Iare Barbalho et Penteado-Dias6
Janzenia Marsh1
Leluthia Cameron1
Lissopsius Marsh2
Masonius Marsh2
Monarea* Szépligeti1
Neoheterospilus Belokobylskij3
Nervellius Roman1
Notiospathius Matthews et Marsh5
Odontobracon Cameron2
Ondigus Braet, Barbalhoa et Van Achterberg2
Panama Marsh1
Psenobolus Reinhard2
Ptesimogaster Marsh1
Rhaconotus Ruthe3
Sabinita Belokobylskij, Zaldívar-Riverón et Martínez1
Spathius Nees1
Stenocorse Marsh3
Vanderentiellus Marsh2
Whitfieldiellus Marsh1
Unidentifed4
The reconstructed NJ tree recovered the megadiverse genus as non-monophyletic with respect to and (Fig. 2). The accumulation curve estimations of species richness (Fig. 3) indicates that the number of species that occur in the region has not been reached.
Figure 2.

Neighbour-joining tree obtained from BOLD that includes 883 nucleotide sequences belonging to doryctine specimens. The distance model used was the Kimura 2 Parameter, the marker was COI-5P, and the 1st, 2nd and 3rd codon positions were included. The sequences had a lengrh greater than 200bp. See Suppl. material 1.

Figure 3.

DNA barcoding species accumulation curve for the from the CBS (Suppl. material 1).

Twenty new species and two new genera ( Belokobylskij, Zaldívar-Riverón et Martínez, Martínez, Belokobylskij et Zaldívar-Riverón) have been described by AZR and collaborators from the doryctine specimens collected for this study (Table 3). An update on Zaldívar-Riverón et al. 2010) generic identification was performed. Specimens assigned to Marsh were transferred to , since the former genus is now considered its junior synonym (De Jesús-Bonilla et al. 2011). Moreover, the specimens assigned in the above study to Marsh and Barbalho et Penteado-Dias actually belong to and , respectively.
Table 3.

List of the new species described from specimens collected in this study

SPECIESREFERENCE
Allorhogas coccolobae Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas crassifemur Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas jaliscoensis Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas marshi Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas parvus Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Allorhogas scotti Martínez and Zaldívar-Riverón Martínez and Zaldívar-Riverón 2013
Ficobolus jaliscoi Zaldívar-Riverón and BelokobylskijMartínez and Zaldívar-Riverón 2013, Zaldívar-Riverón et al. 2014
Heerz ecmahla Martínez, Zaldívar-Riverón, Ceccarelli and Shaw Zaldivar-Riveron et al. 2012
Heerz macrophthalma Martínez, Zaldívar-Riverón, Ceccarelli and Shaw Zaldivar-Riveron et al. 2012
Iare cheguevarai Martínez, Zaldívar-Riverón and Ceccarelli Martínez et al. 2010
Iare mexicanus Martínez, Zaldívar-Riverón and Ceccarelli Martínez et al. 2010
Lissopsius jaliscoensis Zaldívar-Riverón, Martínez, Ceccarelli et Shaw Zaldivar-Riveron et al. 2012
Lissopsius pacificus Zaldívar-Riverón, Martínez, Ceccarelli et Shaw Zaldivar-Riveron et al. 2012
Monarea fridae Belokobylskij, Zaldivar-Riveron et Coronado-Blanco Belokobyskij et al. 2014
Neoheterospilus chamelae Martínez et Zaldívar-Riverón Martínez and Zaldívar-Riverón 2010
Notiospathius crypticus Reséndiz-Flores, Nunes and Zaldívar-Riverón Reséndiz-Flores et al. 2014
Notiospathius mariachi Reséndiz-Flores, Nunes and Zaldívar-Riverón Reséndiz-Flores et al. 2014
Ondigus cuixmalensis Zaldívar-Riverón, Martínez, Ceccarelli and Shaw Zaldivar-Riveron et al. 2012
Sabinita mexicana Belokobylskij, Zaldívar-Riverón and Martínez Zaldívar-Riverón et al. 2014
Spathius chamelae Belokobylskij and Zaldívar-Riverón Belokobylskij and Zaldivar-Riveron 2014

Discussion

The Mexican dry tropical forest is known for containing a considerably high species richness and endemicity rates for various plant and animal groups (Ceballos et al. 2010). Despite that the current available information does not allow to determine what proportion of the richness registered for insects in Mexico occurs in dry tropical forests, the gathered data for other taxa suggest that it is considerably high (Zaragoza-Caballero et al. 2010). This work represents one of various DNA barcoding species inventories that are being carried out for selected braconid subfamilies (, , ) and for other insect taxa (e.g. : , ) in the CBS, one of which has already been published (, Fernández-Flores et al. 2013). Our updated study identified 14 additional genera and increased 53% the number of barcoding species found in the CBS with respect to the results obtained in Zaldívar-Riverón et al. 2010 preliminary study. Though most of the doryctine species that were discriminated remain undescribed, 20 of them and two new genera (Sabinita and Ficobolus) were already described by AZR and collaborators based on the material collected in this work. Most of these new described species belong to small, poorly known genera. The published records for the Chamela region indicate that the species richness of the is considerably higher than the those observed for (103 spp.; Fernández-Flores et al. 2013), considered to be the second largest subfamily of (Jones et al. 2009), and (27 spp.; Aguilar-Velasco 2013). A vast species richness found for the subfamily was reported for Costa Rica (458 spp.; Marsh 2002, Marsh et al. 2013). This species richness, however, was reported for the whole country, whereas in our study the species richness found for the subfamily (290 spp.) was limited to about 3,000 ha. Particularly, we delimited 170 barcoding species for the genus for the CBS, whereas for Costa Rica, Marsh et al. 2013 described 280 species (Marsh et al. 2013). Similar to our preliminary study, paraphyly of with respect to and was again recovered. A non-monophyletic was also recovered in a recent multi locus phylogenetic study (Wild et al. 2013). Our results therefore support that, though it is not appropriate to reconstruct phylogenetic relationships only based on a single mitochondrial marker, barcoding data represents an accessible, comprehensive system for species identification (Hebert and Gregory 2005). Accumulation Curve Data Data type: Table of records Brief description: Table containing the Process ID of specimens sampled and Barcode Index Number (BIN) used for the species accumulation curve. File: oo_40543.xls
  15 in total

1.  Biological identifications through DNA barcodes.

Authors:  Paul D N Hebert; Alina Cywinska; Shelley L Ball; Jeremy R deWaard
Journal:  Proc Biol Sci       Date:  2003-02-07       Impact factor: 5.349

2.  The promise of DNA barcoding for taxonomy.

Authors:  Paul D N Hebert; T Ryan Gregory
Journal:  Syst Biol       Date:  2005-10       Impact factor: 15.683

3.  Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections.

Authors:  M Alex Smith; Josephine J Rodriguez; James B Whitfield; Andrew R Deans; Daniel H Janzen; Winnie Hallwachs; Paul D N Hebert
Journal:  Proc Natl Acad Sci U S A       Date:  2008-08-20       Impact factor: 11.205

4.  DNA barcoding species inventory of Microgastrinae wasps (Hymenoptera, Braconidae) from a Mexican tropical dry forest.

Authors:  S Fernández-Flores; J L Fernández-Triana; J J Martínez; A Zaldívar-Riverón
Journal:  Mol Ecol Resour       Date:  2013-04-17       Impact factor: 7.090

5.  Phylogenetic affinities of Monarea Szépligeti, 1904 (Hymenoptera: Braconidae, Doryctinae, with description of a new species from Mexico.

Authors:  Sergey A Belokobylskij; Alejandro Zaldívar-Riverón; Juana M Coronado-Blanco
Journal:  Zootaxa       Date:  2014-05-14       Impact factor: 1.091

6.  DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.

Authors:  O Folmer; M Black; W Hoeh; R Lutz; R Vrijenhoek
Journal:  Mol Mar Biol Biotechnol       Date:  1994-10

7.  Five new species of the genera Heerz Marsh, Lissopsius Marsh and Ondigus Braet, Barbalho and van Achterberg (Braconidae, Doryctinae) from the Chamela-Cuixmala biosphere reserve in Jalisco, Mexico.

Authors:  Alejandro Zaldívar-Riverón; Juan José Martínez; Fadia Sara Ceccarelli; Scott R Shaw
Journal:  Zookeys       Date:  2012-01-11       Impact factor: 1.546

8.  The genus Spathius Nees (Hymenoptera, Braconidae, Doryctinae) in Mexico: occurrence of a highly diverse Old World taxon in the Neotropics.

Authors:  Sergey A Belokobylskij; Alejandro Zaldívar-Riverón
Journal:  Zookeys       Date:  2014-07-21       Impact factor: 1.546

9.  A DNA-based registry for all animal species: the barcode index number (BIN) system.

Authors:  Sujeevan Ratnasingham; Paul D N Hebert
Journal:  PLoS One       Date:  2013-07-08       Impact factor: 3.240

10.  Fast-evolving homoplastic traits are best for species identification in a group of neotropical wasps.

Authors:  Alexander L Wild; Paul M Marsh; James B Whitfield
Journal:  PLoS One       Date:  2013-09-11       Impact factor: 3.240
View more
  2 in total

1.  Preliminary Investigation of Species Diversity of Rice Hopper Parasitoids in Southeast Asia.

Authors:  Christina Sann; Franziska Wemheuer; Alexis Beaurepaire; Rolf Daniel; Silvio Erler; Stefan Vidal
Journal:  Insects       Date:  2018-02-09       Impact factor: 2.769

2.  Transcriptome profiling of venom gland from wasp species: de novo assembly, functional annotation, and discovery of molecular markers.

Authors:  Junjie Tan; Wenbo Wang; Fan Wu; Yunming Li; Quanshui Fan
Journal:  BMC Genomics       Date:  2020-06-24       Impact factor: 3.969
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.