Literature DB >> 26021570

A series of medium and high copy number arabinose-inducible Escherichia coli expression vectors compatible with pBR322 and pACYC184.

Vandana Chakravartty1, John E Cronan2.   

Abstract

The original pBAD24 plasmid and the derived lower copy number (the pBAD322 series) expression vectors have been widely used in Escherichia coli, Salmonella enterica, and related bacteria. However, a flexible pBAD expression system has been available only in pMB1 (ColE1) vectors. We report a series of pBAD vectors that replicate using the origin of plasmid RSF1030 that are compatible with pMB1 (ColE1) and p15A (pACYC) vectors. Both high (≥pBAD24) and medium (~pBAD322) copy number plasmids encoding resistance to ampicillin, chloramphenicol, kanamycin, tetracycline, spectinomycin/streptomycin, gentamycin, or trimethoprim are available.
Copyright © 2015 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Copy number; Expression levels; Plasmid Compatibility; RSF1030; pBAD24; pBR322

Mesh：

Substances：

Year:  2015        PMID： 26021570      PMCID： PMC4600428          DOI： 10.1016/j.plasmid.2015.03.001

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  24 in total

1.  High copy number plasmids compatible with commonly used cloning vectors.

Authors:  G J Phillips; S K Park; D Huber
Journal:  Biotechniques       Date:  2000-03       Impact factor: 1.993

2.  A family of arabinose-inducible Escherichia coli expression vectors having pBR322 copy control.

Authors:  John E Cronan
Journal:  Plasmid       Date:  2005-08-31       Impact factor: 3.466

3.  One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.

Authors:  K A Datsenko; B L Wanner
Journal:  Proc Natl Acad Sci U S A       Date:  2000-06-06       Impact factor: 11.205

4.  Improved antibiotic-resistance cassettes through restriction site elimination using Pfu DNA polymerase PCR.

Authors:  J J Dennis; G J Zylstra
Journal:  Biotechniques       Date:  1998-11       Impact factor: 1.993

Review 5.  Polyadenylation of mRNA in bacteria.

Authors:  N Sarkar
Journal:  Microbiology       Date:  1996-11       Impact factor: 2.777

6.  Evidence against translational repression by the carboxyltransferase component of Escherichia coli acetyl coenzyme A carboxylase.

Authors:  Alexander C Smith; John E Cronan
Journal:  J Bacteriol       Date:  2014-08-25       Impact factor: 3.490

Review 7.  Ribonucleases, antisense RNAs and the control of bacterial plasmids.

Authors:  Margarida Saramago; Cátia Bárria; Cecília M Arraiano; Susana Domingues
Journal:  Plasmid       Date:  2014-09-28       Impact factor: 3.466

8.  Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.

Authors:  L M Guzman; D Belin; M J Carson; J Beckwith
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

9.  Role of the rom protein in copy number control of plasmid pBR322 at different growth rates in Escherichia coli K-12.

Authors:  T Atlung; B B Christensen; F G Hansen
Journal:  Plasmid       Date:  1999-03       Impact factor: 3.466

10.  Construction and use of low-copy number T7 expression vectors for purification of problem proteins: purification of mycobacterium tuberculosis RmlD and pseudomonas aeruginosa LasI and RhlI proteins, and functional analysis of purified RhlI.

Authors:  T T Hoang; Y Ma; R J Stern; M R McNeil; H P Schweizer
Journal:  Gene       Date:  1999-09-17       Impact factor: 3.688
View more
  6 in total

1.  Beyond Plug and Pray: Context Sensitivity and in silico Design of Artificial Neomycin Riboswitches.

Authors:  Christian Günzel; Felix Kühnl; Katharina Arnold; Sven Findeiß; Christina E Weinberg; Peter F Stadler; Mario Mörl
Journal:  RNA Biol       Date:  2020-10-25       Impact factor: 4.652

2.  Escherichia coli vectors having stringently repressible replication origins allow a streamlining of Crispr/Cas9 gene editing.

Authors:  Swaminath Srinivas; Zhe Hu; John E Cronan
Journal:  Plasmid       Date:  2019-04-29       Impact factor: 3.466

3.  pBR322 vectors having tetracycline-dependent replication.

Authors:  John E Cronan
Journal:  Plasmid       Date:  2016-02-11       Impact factor: 3.466

4.  Protein moonlighting elucidates the essential human pathway catalyzing lipoic acid assembly on its cognate enzymes.

Authors:  Xinyun Cao; Lei Zhu; Xuejiao Song; Zhe Hu; John E Cronan
Journal:  Proc Natl Acad Sci U S A       Date:  2018-07-09       Impact factor: 11.205

5.  Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit.

Authors:  Qiang Tang; Chunbo Lou; Shuang-Jiang Liu
Journal:  J Biol Eng       Date:  2017-09-04       Impact factor: 4.355

6.  Improving an Escherichia coli-based biocatalyst for terpenol glycosylation by variation of the expression system.

Authors:  Julian Rüdiger; Wilfried Schwab
Journal:  J Ind Microbiol Biotechnol       Date:  2019-05-06       Impact factor: 3.346
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.