CONTEXT: MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression. In white adipose tissue (WAT), recent studies suggest that miRNA levels are altered in various metabolic diseases, including obesity. OBJECTIVE: The objective of the study was to determine whether adipocyte-expressed miRNAs altered by obesity can regulate adiponectin expression/secretion in fat cells. DESIGN: Eleven miRNAs previously shown to be altered in obese human WAT were overexpressed in human in vitro-differentiated adipocytes followed by assessments of adiponectin levels in conditioned media. SETTING: This was cohort study (n = 56) in an academic hospital. PATIENTS: Subcutaneous WAT was obtained from nonobese and obese individuals. INTERVENTIONS: There were no interventions in this study. MAIN OUTCOME MEASURE(S): Protein and mRNA levels of adiponectin were measured. RESULTS: Of the 11 investigated miRNAs, three (miR-193b/-126/-26a) increased adiponectin secretion when overexpressed in human adipocytes. However, in human WAT only miR-193b expression correlated with adiponectin gene expression and homeostasis model assessment of insulin resistance. Moreover, quantitative PCR of miR-193b in both WAT and isolated adipocytes showed a significant association with serum adiponectin levels. Overexpression of miR-193b altered the gene expression of seven known adiponectin regulators. 3'-untranslated region reporter assays confirmed binding to cAMP-responsive element binding protein 5, nuclear receptor interacting protein 1, and nuclear transcription factor Yα. The effects of miR-193b on nuclear transcription factor Yα expression were confirmed at the protein level. Transfection with individual miRNA target protectors selective for nuclear transcription factor Yα and nuclear receptor interacting protein 1 abolished the stimulatory effect of miR-193b on adiponectin secretion. CONCLUSIONS: In human adipocytes, miR-193b controls adiponectin production via pathways involving nuclear transcription factor Yα and possibly nuclear receptor interacting protein 1.
CONTEXT: MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression. In white adipose tissue (WAT), recent studies suggest that miRNA levels are altered in various metabolic diseases, including obesity. OBJECTIVE: The objective of the study was to determine whether adipocyte-expressed miRNAs altered by obesity can regulate adiponectin expression/secretion in fat cells. DESIGN: Eleven miRNAs previously shown to be altered in obesehuman WAT were overexpressed in human in vitro-differentiated adipocytes followed by assessments of adiponectin levels in conditioned media. SETTING: This was cohort study (n = 56) in an academic hospital. PATIENTS: Subcutaneous WAT was obtained from nonobese and obese individuals. INTERVENTIONS: There were no interventions in this study. MAIN OUTCOME MEASURE(S): Protein and mRNA levels of adiponectin were measured. RESULTS: Of the 11 investigated miRNAs, three (miR-193b/-126/-26a) increased adiponectin secretion when overexpressed in human adipocytes. However, in human WAT only miR-193b expression correlated with adiponectin gene expression and homeostasis model assessment of insulin resistance. Moreover, quantitative PCR of miR-193b in both WAT and isolated adipocytes showed a significant association with serum adiponectin levels. Overexpression of miR-193b altered the gene expression of seven known adiponectin regulators. 3'-untranslated region reporter assays confirmed binding to cAMP-responsive element binding protein 5, nuclear receptor interacting protein 1, and nuclear transcription factor Yα. The effects of miR-193b on nuclear transcription factor Yα expression were confirmed at the protein level. Transfection with individual miRNA target protectors selective for nuclear transcription factor Yα and nuclear receptor interacting protein 1 abolished the stimulatory effect of miR-193b on adiponectin secretion. CONCLUSIONS: In human adipocytes, miR-193b controls adiponectin production via pathways involving nuclear transcription factor Yα and possibly nuclear receptor interacting protein 1.
Authors: Taijyu Satoh; Longfei Wang; Cristina Espinosa-Diez; Bing Wang; Scott A Hahn; Kentaro Noda; Elizabeth R Rochon; Matthew R Dent; Andrea R Levine; Jeffrey J Baust; Samuel Wyman; Yijen L Wu; Georgios A Triantafyllou; Ying Tang; Mike Reynolds; Sruti Shiva; Cynthia St Hilaire; Delphine Gomez; Dmitry A Goncharov; Elena A Goncharova; Stephen Y Chan; Adam C Straub; Yen-Chun Lai; Charles F McTiernan; Mark T Gladwin Journal: Circulation Date: 2021-06-23 Impact factor: 39.918
Authors: Xiaoxiao Peng; Alfredo Giménez-Cassina; Paul Petrus; Marcus Conrad; Mikael Rydén; Elias S J Arnér Journal: Sci Rep Date: 2016-06-27 Impact factor: 4.379