OBJECTIVES: Leukocyte NADPH oxidase, which is active in neutrophils, is a membrane-bound enzyme that catalyzes the reduction of oxygen to O2(-) by using NADPH as an electron donor. Previously, we reported that casein kinase 2 (CK2), a ubiquitous and highly conserved Ser/Thr kinase, is responsible for p47(phox) phosphorylation and that phosphorylation of p47(phox) by CK2 regulates the deactivation of NADPH oxidase. METHODS: Here, we report that the residue Cys(196) of p47(phox) is a target of S-nitrosylation by S-nitrosothiol and peroxynitrite and that this modification enhanced phosphorylation of p47(phox) by CK2. RESULTS: S-Nitrosylated p47(phox) enhanced CK2 b subunit binding, presumably due to alterations in protein conformation. DISCUSSION: Taken together, we propose that S-nitrosylation of p47(phox) regulates the deactivation of NADPH oxidase via enhancement of p47(phox) phosphorylation by CK2.
OBJECTIVES: Leukocyte NADPH oxidase, which is active in neutrophils, is a membrane-bound enzyme that catalyzes the reduction of oxygen to O2(-) by using NADPH as an electron donor. Previously, we reported that casein kinase 2 (CK2), a ubiquitous and highly conserved Ser/Thr kinase, is responsible for p47(phox) phosphorylation and that phosphorylation of p47(phox) by CK2 regulates the deactivation of NADPH oxidase. METHODS: Here, we report that the residue Cys(196) of p47(phox) is a target of S-nitrosylation by S-nitrosothiol and peroxynitrite and that this modification enhanced phosphorylation of p47(phox) by CK2. RESULTS: S-Nitrosylated p47(phox) enhanced CK2 b subunit binding, presumably due to alterations in protein conformation. DISCUSSION: Taken together, we propose that S-nitrosylation of p47(phox) regulates the deactivation of NADPH oxidase via enhancement of p47(phox) phosphorylation by CK2.
Authors: Stavros Selemidis; Gregory J Dusting; Hitesh Peshavariya; Barbara K Kemp-Harper; Grant R Drummond Journal: Cardiovasc Res Date: 2007-04-21 Impact factor: 10.787
Authors: Ayumi Hashimoto; Chan Gao; Jerome Mastio; Andrew Kossenkov; Scott I Abrams; Ashok V Purandare; Heshani Desilva; Susan Wee; John Hunt; Maria Jure-Kunkel; Dmitry I Gabrilovich Journal: Cancer Res Date: 2018-08-23 Impact factor: 12.701
Authors: Christa Zollbrecht; A Erik G Persson; Jon O Lundberg; Eddie Weitzberg; Mattias Carlström Journal: Redox Biol Date: 2016-09-28 Impact factor: 11.799