OBJECTIVE: Accumulating evidences suggest that pro-inflammatory cytokines such as interleukin-6 (IL-6) play a role in the pathophysiology of depression. In the learned helplessness (LH) paradigm, ~35% rats are resilient to inescapable stress. METHODS: Levels of IL-6 in the serum and medial prefrontal cortex (mPFC) of LH rats (susceptible) and non-LH rats (resilience) were measured using enzyme-linked immunosorbent assay and western blot analysis, respectively. RESULTS: Serum levels of IL-6 in the LH rats were significantly higher than those of control and non-LH rats. In contrast, tissue levels of IL-6 in the mPFC were not different among three groups. CONCLUSION: The results suggest that peripheral IL-6 may contribute to resilience versus susceptibility to inescapable stress.
OBJECTIVE: Accumulating evidences suggest that pro-inflammatory cytokines such as interleukin-6 (IL-6) play a role in the pathophysiology of depression. In the learned helplessness (LH) paradigm, ~35% rats are resilient to inescapable stress. METHODS: Levels of IL-6 in the serum and medial prefrontal cortex (mPFC) of LH rats (susceptible) and non-LH rats (resilience) were measured using enzyme-linked immunosorbent assay and western blot analysis, respectively. RESULTS: Serum levels of IL-6 in the LH rats were significantly higher than those of control and non-LH rats. In contrast, tissue levels of IL-6 in the mPFC were not different among three groups. CONCLUSION: The results suggest that peripheral IL-6 may contribute to resilience versus susceptibility to inescapable stress.
Authors: Qian Ren; Min Ma; Tamaki Ishima; Christophe Morisseau; Jun Yang; Karen M Wagner; Ji-Chun Zhang; Chun Yang; Wei Yao; Chao Dong; Mei Han; Bruce D Hammock; Kenji Hashimoto Journal: Proc Natl Acad Sci U S A Date: 2016-03-14 Impact factor: 11.205
Authors: Kelsey M Loupy; Kristin E Cler; Brandon M Marquart; Tumim W Yifru; Heather M D'Angelo; Mathew R Arnold; Ahmed I Elsayed; Matthew J Gebert; Noah Fierer; Laura K Fonken; Matthew G Frank; Cristian A Zambrano; Steven F Maier; Christopher A Lowry Journal: Brain Behav Immun Date: 2020-10-01 Impact factor: 7.217