| Literature DB >> 26017769 |
Michael R M Harrison1, Jeroen Bussmann2, Ying Huang1, Long Zhao3, Arthela Osorio1, C Geoffrey Burns3, Caroline E Burns3, Henry M Sucov4, Arndt F Siekmann2, Ching-Ling Lien5.
Abstract
Interruption of the coronary blood supply severely impairs heart function with often fatal consequences for patients. However, the formation and maturation of these coronary vessels is not fully understood. Here we provide a detailed analysis of coronary vessel development in zebrafish. We observe that coronary vessels form in zebrafish by angiogenic sprouting of arterial cells derived from the endocardium at the atrioventricular canal. Endothelial cells express the CXC-motif chemokine receptor Cxcr4a and migrate to vascularize the ventricle under the guidance of the myocardium-expressed ligand Cxcl12b. cxcr4a mutant zebrafish fail to form a vascular network, whereas ectopic expression of Cxcl12b ligand induces coronary vessel formation. Importantly, cxcr4a mutant zebrafish fail to undergo heart regeneration following injury. Our results suggest that chemokine signaling has an essential role in coronary vessel formation by directing migration of endocardium-derived endothelial cells. Poorly developed vasculature in cxcr4a mutants likely underlies decreased regenerative potential in adults.Entities:
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Year: 2015 PMID： 26017769 PMCID： PMC4448080 DOI： 10.1016/j.devcel.2015.04.001
Source DB: PubMed Journal: Dev Cell ISSN： 1534-5807 Impact factor: 12.270