Jelena Kotur-Stevuljevic1, Natasa Bogavac-Stanojevic2, Zorana Jelic-Ivanovic3, Aleksandra Stefanovic4, Tamara Gojkovic5, Jelena Joksic6, Miron Sopic7, Brankica Gulan8, Jelena Janac9, Sanja Milosevic10. 1. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: jkotur@pharmacy.bg.ac.rs. 2. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: naca@pharmacy.bg.ac.rs. 3. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: zorana.jelic@pharmacy.bg.ac.rs. 4. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: alex@pharmacy.bg.ac.rs. 5. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: gojkotamara@gmail.com. 6. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: jelenajoksic@yahoo.com. 7. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: miron@pharmacy.bg.ac.rs. 8. Special Hospital for Prevention and Therapy of Cerebrovascular Disease "Sveti Sava", Nemanjina 2, 11000 Belgrade, Serbia. Electronic address: gulan_b@hotmail.com. 9. Institute of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: jelena.janac@gmail.com. 10. Health Center, Hospital Zaječar, Rasadnička b.b., 19000 Zaječar, Serbia. Electronic address: jkotur69@gmail.com.
Abstract
OBJECTIVE: The connection of oxidative stress with dyslipidemia creates a newly-emerging atherosclerosis risk factor involved in acute ischemic stroke development. This study analyzed the influence of oxidative stress on structural changes of high-density lipoprotein (HDL) particles connected with modification in protective paraoxonase 1 (PON1) activity. METHODS: This study used 185 patients with acute ischemic stroke and 185 apparently healthy controls. Oxidative stress status, PON1 status, lipids and high-sensitivity C-reactive protein (hsCRP) were determined. In isolated HDL lipoprotein fraction we determined selected markers of oxidative stress (malondialdehyde, MDA) and the content of total sulfhydryl (SH) groups. The capability of oxidative and PON1 status parameters to discriminate patients according to survival status was evaluated. RESULTS: Stroke patients had lower HDL-cholesterol than controls and a remarkable fall in PON1 activity (control group-227U/L, survivors-42U/L, lethal outcome group-61U/L, p < 0.001), along with more prominent inflammation. Pronounced oxidative stress and impaired antioxidative protection was present among patients. HDL fraction analysis revealed a significant decrease of SH groups content (control group vs. patients, p < 0.05) and increased in MDA content in patients (lethal outcome vs. control group, p < 0.05). According to logistic regression analysis, the best predictor of disease outcome was oxidative stress marker - prooxidative-antioxidative balance (PAB). CONCLUSIONS: Pronounced oxidative stress in this group of acute ischemic stroke patients probably led to HDL structural changes, which could further cause an alteration or decrease of PON1 activity. Evidence of increased prooxidant level associated with decreased protective, antioxidative factors suggests their mutual involvement in this complex pathology.
OBJECTIVE: The connection of oxidative stress with dyslipidemia creates a newly-emerging atherosclerosis risk factor involved in acute ischemic stroke development. This study analyzed the influence of oxidative stress on structural changes of high-density lipoprotein (HDL) particles connected with modification in protective paraoxonase 1 (PON1) activity. METHODS: This study used 185 patients with acute ischemic stroke and 185 apparently healthy controls. Oxidative stress status, PON1 status, lipids and high-sensitivity C-reactive protein (hsCRP) were determined. In isolated HDL lipoprotein fraction we determined selected markers of oxidative stress (malondialdehyde, MDA) and the content of total sulfhydryl (SH) groups. The capability of oxidative and PON1 status parameters to discriminate patients according to survival status was evaluated. RESULTS:Strokepatients had lower HDL-cholesterol than controls and a remarkable fall in PON1 activity (control group-227U/L, survivors-42U/L, lethal outcome group-61U/L, p < 0.001), along with more prominent inflammation. Pronounced oxidative stress and impaired antioxidative protection was present among patients. HDL fraction analysis revealed a significant decrease of SH groups content (control group vs. patients, p < 0.05) and increased in MDA content in patients (lethal outcome vs. control group, p < 0.05). According to logistic regression analysis, the best predictor of disease outcome was oxidative stress marker - prooxidative-antioxidative balance (PAB). CONCLUSIONS: Pronounced oxidative stress in this group of acute ischemic strokepatients probably led to HDL structural changes, which could further cause an alteration or decrease of PON1 activity. Evidence of increased prooxidant level associated with decreased protective, antioxidative factors suggests their mutual involvement in this complex pathology.
Authors: Samantha A Reina; Maria M Llabre; Matthew A Allison; John T Wilkins; Armando J Mendez; Martinson K Arnan; Neil Schneiderman; Ralph L Sacco; Mercedes Carnethon; J A Chris Delaney Journal: Atherosclerosis Date: 2015-09-28 Impact factor: 5.162
Authors: Slawomir Michalak; Wojciech Ambrosius; Ewa Wysocka; Mieczyslaw Dziarmaga; Robert Juszkat; Andrzej Wykretowicz; Wojciech Kozubski Journal: Oxid Med Cell Longev Date: 2016-12-13 Impact factor: 6.543
Authors: Stine K Venø; Christian S Bork; Marianne U Jakobsen; Søren Lundbye-Christensen; Flemming W Bach; Peter L McLennan; Anne Tjønneland; Erik B Schmidt; Kim Overvad Journal: Nutrients Date: 2018-11-03 Impact factor: 5.717