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Literature DB >> 26013980

Is mouse embryonic stem cell technology obsolete?

Abstract

Injection of recombinant Cas9 protein and synthetic guide RNAs into mouse zygotes has been shown to facilitate gene disruption and knock-ins using the CRISPR system. These technologies may soon displace genetic modification using embryonic stem cells.

Entities: Chemical Disease Gene Species

Mesh：

Year: 2015 PMID： 26013980 PMCID： PMC4443626 DOI： 10.1186/s13059-015-0673-6

Source DB: PubMed Journal: Genome Biol ISSN： 1474-7596 Impact factor: 13.583

10 in total

1. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering.

Authors: Hui Yang; Haoyi Wang; Chikdu S Shivalila; Albert W Cheng; Linyu Shi; Rudolf Jaenisch
Journal: Cell Date: 2013-08-29 Impact factor: 41.582

2. A conditional knockout resource for the genome-wide study of mouse gene function.

Authors: William C Skarnes; Barry Rosen; Anthony P West; Manousos Koutsourakis; Wendy Bushell; Vivek Iyer; Alejandro O Mujica; Mark Thomas; Jennifer Harrow; Tony Cox; David Jackson; Jessica Severin; Patrick Biggs; Jun Fu; Michael Nefedov; Pieter J de Jong; A Francis Stewart; Allan Bradley
Journal: Nature Date: 2011-06-15 Impact factor: 49.962

3. Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases.

Authors: Melanie Meyer; Martin Hrabé de Angelis; Wolfgang Wurst; Ralf Kühn
Journal: Proc Natl Acad Sci U S A Date: 2010-08-04 Impact factor: 11.205

4. Double-strand breaks at the target locus stimulate gene targeting in embryonic stem cells.

Authors: F Smih; P Rouet; P J Romanienko; M Jasin
Journal: Nucleic Acids Res Date: 1995-12-25 Impact factor: 16.971

5. Targeted correction of a major histocompatibility class II E alpha gene by DNA microinjected into mouse eggs.

Authors: R L Brinster; R E Braun; D Lo; M R Avarbock; F Oram; R D Palmiter
Journal: Proc Natl Acad Sci U S A Date: 1989-09 Impact factor: 11.205

6. Extensive genomic copy number variation in embryonic stem cells.

Authors: Qi Liang; Nathalie Conte; William C Skarnes; Allan Bradley
Journal: Proc Natl Acad Sci U S A Date: 2008-11-06 Impact factor: 11.205

7. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.

Authors: Haoyi Wang; Hui Yang; Chikdu S Shivalila; Meelad M Dawlaty; Albert W Cheng; Feng Zhang; Rudolf Jaenisch
Journal: Cell Date: 2013-05-02 Impact factor: 41.582

8. Mice with megabase humanization of their immunoglobulin genes generate antibodies as efficiently as normal mice.

Authors: Andrew J Murphy; Lynn E Macdonald; Sean Stevens; Margaret Karow; Anthony T Dore; Kevin Pobursky; Tammy T Huang; William T Poueymirou; Lakeisha Esau; Melissa Meola; Warren Mikulka; Pamela Krueger; Jeanette Fairhurst; David M Valenzuela; Nicholas Papadopoulos; George D Yancopoulos
Journal: Proc Natl Acad Sci U S A Date: 2014-03-25 Impact factor: 11.205

9. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice.

Authors: Tomomi Aida; Keiho Chiyo; Takako Usami; Harumi Ishikubo; Risa Imahashi; Yusaku Wada; Kenji F Tanaka; Tetsushi Sakuma; Takashi Yamamoto; Kohichi Tanaka
Journal: Genome Biol Date: 2015-04-29 Impact factor: 13.583

10. Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases.

Authors: Young Hoon Sung; Jong Min Kim; Hyun-Taek Kim; Jaehoon Lee; Jisun Jeon; Young Jin; Jung-Hwa Choi; Young Ho Ban; Sang-Jun Ha; Cheol-Hee Kim; Han-Woong Lee; Jin-Soo Kim
Journal: Genome Res Date: 2013-11-19 Impact factor: 9.043

10 in total

9 in total

Is mouse embryonic stem cell technology obsolete?

1. One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering.

2. A conditional knockout resource for the genome-wide study of mouse gene function.

3. Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases.

4. Double-strand breaks at the target locus stimulate gene targeting in embryonic stem cells.

5. Targeted correction of a major histocompatibility class II E alpha gene by DNA microinjected into mouse eggs.

6. Extensive genomic copy number variation in embryonic stem cells.

7. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering.

8. Mice with megabase humanization of their immunoglobulin genes generate antibodies as efficiently as normal mice.

9. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice.

10. Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases.

1. GONAD: A Novel CRISPR/Cas9 Genome Editing Method that Does Not Require Ex Vivo Handling of Embryos.

2. Easi-CRISPR for creating knock-in and conditional knockout mouse models using long ssDNA donors.

Review 3. Application of Mouse Models to Research in Hearing and Balance.

4. Efficient mouse genome engineering by CRISPR-EZ technology.

5. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ.

Review 6. Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation.

7. Maternal Supply of Cas9 to Zygotes Facilitates the Efficient Generation of Site-Specific Mutant Mouse Models.

Review 8. Generating mouse models for biomedical research: technological advances.

9. Generation of mouse conditional knockout alleles in one step using the i-GONAD method.