Anastasia Tsigkou1, Fernando M Reis2, Meng H Lee1, Bingjie Jiang3, Claudia Tosti4, Gabriele Centini4, Fang-Rong Shen5, You-Guo Chen5, Felice Petraglia6. 1. Department of Biological Sciences, Xi'an Jiaotong Liverpool University, Suzhou, People's Republic of China. 2. Division of Human Reproduction, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. 3. Guangzhou University, Panyu, People's Republic of China. 4. Department of Molecular and Developmental Medicine, Obstetrics and Gynecology, University of Siena, Siena, Italy. 5. Department of Obstetrics and Gynecology, First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China. 6. Department of Molecular and Developmental Medicine, Obstetrics and Gynecology, University of Siena, Siena, Italy. Electronic address: felice.petraglia@unisi.it.
Abstract
OBJECTIVE: To investigate the possible correlation between progesterone receptor (PR) expression in uterine leiomyoma or adjacent myometrium and patient's age, size/number of leiomyomas, or clinical symptoms such as dysmenorrhea, acyclic pelvic pain, or menstrual and intermenstrual uterine bleeding. DESIGN: Cross-sectional study. SETTING: Referral center. PATIENT(S): Sixty-two Chinese women undergoing elective hysterectomy for uterine leiomyomata. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Evaluation of PR-total and PR-B mRNA with real-time polymerase chain reaction; PR-A and PR-B proteins quantified by Western blot in leiomyoma tissue and myometrium; symptoms rated by the patients using visual analog scores. RESULT(S): The PR-B mRNA and PR-A and PR-B proteins were more concentrated in leiomyomas than in matched myometrium. A direct correlation between PR-B mRNA levels in leiomyoma and age (r = 0.347) and number of tumors (r = 0.295) was found. Conversely, there was an inverse correlation between PR-B mRNA levels in leiomyoma and dysmenorrhea (r = -0.260) and intermenstrual bleeding (r = -0.266). Multiple regression analysis indicated that age (β = 0.363) and the number of myomas (β = 0.296) were independently associated with PR-B mRNA levels in leiomyoma tissue. CONCLUSION(S): The levels of PR-B mRNA in leiomyoma tissue are directly associated with the number of tumors and inversely correlated with the intensity of intermenstrual bleeding and dysmenorrhea, suggesting that PR signaling may favor leiomyoma growth while attenuating clinical symptoms. This duality should be taken into account in the clinical management of patients with symptomatic uterine leiomyoma.
OBJECTIVE: To investigate the possible correlation between progesterone receptor (PR) expression in uterine leiomyoma or adjacent myometrium and patient's age, size/number of leiomyomas, or clinical symptoms such as dysmenorrhea, acyclic pelvic pain, or menstrual and intermenstrual uterine bleeding. DESIGN: Cross-sectional study. SETTING: Referral center. PATIENT(S): Sixty-two Chinese women undergoing elective hysterectomy for uterine leiomyomata. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Evaluation of PR-total and PR-B mRNA with real-time polymerase chain reaction; PR-A and PR-B proteins quantified by Western blot in leiomyoma tissue and myometrium; symptoms rated by the patients using visual analog scores. RESULT(S): The PR-B mRNA and PR-A and PR-B proteins were more concentrated in leiomyomas than in matched myometrium. A direct correlation between PR-B mRNA levels in leiomyoma and age (r = 0.347) and number of tumors (r = 0.295) was found. Conversely, there was an inverse correlation between PR-B mRNA levels in leiomyoma and dysmenorrhea (r = -0.260) and intermenstrual bleeding (r = -0.266). Multiple regression analysis indicated that age (β = 0.363) and the number of myomas (β = 0.296) were independently associated with PR-B mRNA levels in leiomyoma tissue. CONCLUSION(S): The levels of PR-B mRNA in leiomyoma tissue are directly associated with the number of tumors and inversely correlated with the intensity of intermenstrual bleeding and dysmenorrhea, suggesting that PR signaling may favor leiomyoma growth while attenuating clinical symptoms. This duality should be taken into account in the clinical management of patients with symptomatic uterine leiomyoma.
Authors: Lan Hai; Maria M Szwarc; Margeaux Wetendorf; San-Pin Wu; Mary C Peavey; Sandra L Grimm; Dean P Edwards; Francesco J DeMayo; John P Lydon Journal: Genesis Date: 2018-08-10 Impact factor: 2.487
Authors: Robert Hermes; Franz Schwarzenberger; Frank Göritz; Serena Oh; Teresa Fernandes; Rui Bernardino; Antoine Leclerc; Eva Greunz; Abraham Mathew; Sarah Forsyth; Joseph Saragusty; Thomas Bernd Hildebrandt Journal: PLoS One Date: 2016-07-12 Impact factor: 3.240