| Literature DB >> 26005898 |
Xiaojun Sun1, Lingyun Li2, Katherine H Overdier, Lee Anne Ammons, Ivor S Douglas, Clay Cothren Burlew, Fuming Zhang, Eric P Schmidt3, Lianli Chi1, Robert J Linhardt.
Abstract
The determination of complex analytes, present at low concentrations, in biological fluids poses a difficult challenge. This study relies on an optimized method of recovery, enzymatic treatment, and disaccharide analysis by liquid chromatography-tandem mass spectrometry to rapidly determine low concentrations of glycosaminoglycans in human urine. The approach utilizes multiple reaction monitoring (MRM) of glycosaminoglycan disaccharides obtained from treating urine samples with recombinant heparin lyases and chondroitin lyase. This rapid and sensitive method allows the analysis of glycosaminoglycan content and disaccharide composition in urine samples having concentrations 10- to 100-fold lower than those typically analyzed from patients with metabolic diseases, such as mucopolysaccharidosis. The current method facilitates the analysis low (ng/mL) levels of urinary glycosaminoglycans present in healthy individuals and in patients with pathological conditions, such as inflammation and cancers, that can subtly alter glycosaminoglycan content and composition.Entities:
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Year: 2015 PMID： 26005898 PMCID： PMC4822829 DOI： 10.1021/acs.analchem.5b00913
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986