| Literature DB >> 26004809 |
Barbara Crescenzi1, Valeria Nofrini1, Gianluca Barba1, Caterina Matteucci1, Danika Di Giacomo1, Paolo Gorello1, Berna Beverloo2, Antonella Vitale3, Iwona Wlodarska4, Peter Vandenberghe4, Roberta La Starza1, Cristina Mecucci5.
Abstract
We assessed lineage involvement by NUP98 translocations in myelodysplastic syndromes (MDS), acute myeloid leukaemia (AML), and T-cell acute lymphoblastic leukaemia (T-ALL). Single cell analysis by FICTION (Fluorescence Immunophenotype and Interphase Cytogenetics as a Tool for Investigation of Neoplasms) showed that, despite diverse partners, i.e. NSD1, DDX10, RAP1GDS1, and LNP1, NUP98 translocations always affected a CD34+/CD133+ hematopoietic precursor. Interestingly the abnormal clone included myelomonocytes, erythroid cells, B- and T- lymphocytes in MDS/AML and only CD7+/CD3+ cells in T-ALL. The NUP98-RAP1GDS1 affected different hematopoietic lineages in AML and T-ALL. Additional specific genomic events, were identified, namely FLT3 and CEBPA mutations in MDS/AML, and NOTCH1 mutations and MYB duplication in T-ALL.Entities:
Keywords: Leukaemia; Lineage affiliation; NUP98 translocations; Single cell analysis
Mesh：
Substances：
Year: 2015 PMID： 26004809 DOI： 10.1016/j.leukres.2015.04.014
Source DB: PubMed Journal: Leuk Res ISSN： 0145-2126 Impact factor: 3.156