| Literature DB >> 2600427 |
H M Cheng1, Y F Ngeow, C K Sam.
Abstract
Heat treatment of sera at 56 degrees C for 30 min results in positive ELISA reactions for anti-cardiolipin antibody (aCL) in sera that had undetectable or low levels of aCL before heat inactivation. The positive, potentiated reactivity of the heated sera in the aCL ELISA could be inhibited with the cardiolipin antigen and was abolished by prior IgG depletion using staphylococcal protein A. The heat-potentiating effect of aCL binding in ELISA was evident in both normal human sera and clinical sera including sera from patients with systemic lupus erythematosus and syphilis.Entities:
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Year: 1989 PMID： 2600427 DOI： 10.1016/0022-1759(89)90359-1
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303