Coralie Germain-Genevois1, Olivia Garandeau2, Franck Couillaud3. 1. Institut de Bio-Imagerie (IBIO), CNRS/UMS 3428, University Bordeaux, 146 rue Léo Saignat, 33076, Bordeaux cedex, France. 2. Centre de Résonance Magnétique des Systèmes Biologiques (RMSB), CNRS/UMR 5536, University Bordeaux, 146 rue Léo Saignat, 33076, Bordeaux cedex, France. 3. Centre de Résonance Magnétique des Systèmes Biologiques (RMSB), CNRS/UMR 5536, University Bordeaux, 146 rue Léo Saignat, 33076, Bordeaux cedex, France. franck.couillaud@u-bordeaux.fr.
Abstract
PURPOSE: Bioluminescence imaging (BLI) is a technique with a low background noise and high sensitivity which is widely used in mice models in oncology. We aimed to assess BLI efficiency of the new luciferase NanoLuc (Nluc) for glioblastoma cell lines and tumors, including for dual reporter applications of deep brain tumors and systemic metastasis when combined with firefly luciferase (Fluc). PROCEDURES: U87 cells were genetically modified for constitutive production of either Nluc, Fluc, or both and assayed for luciferase activity and BLI on cell lysates, living cells, subcutaneous tumors, brain tumors, and systemic metastases. RESULTS: In vitro, light production by Nluc activity is higher than Fluc. In vivo, Nluc allows for tumor detection including for deep brain tumors and systemic metastases. CONCLUSIONS: Nluc appears to be a useful tool to combine with Fluc for dual imaging in vivo using bioluminescence, allowing for the detection of distinct events in deep tissues within the same organism.
PURPOSE: Bioluminescence imaging (BLI) is a technique with a low background noise and high sensitivity which is widely used in mice models in oncology. We aimed to assess BLI efficiency of the new luciferase NanoLuc (Nluc) for glioblastoma cell lines and tumors, including for dual reporter applications of deep brain tumors and systemic metastasis when combined with firefly luciferase (Fluc). PROCEDURES: U87 cells were genetically modified for constitutive production of either Nluc, Fluc, or both and assayed for luciferase activity and BLI on cell lysates, living cells, subcutaneous tumors, brain tumors, and systemic metastases. RESULTS: In vitro, light production by Nluc activity is higher than Fluc. In vivo, Nluc allows for tumor detection including for deep brain tumors and systemic metastases. CONCLUSIONS: Nluc appears to be a useful tool to combine with Fluc for dual imaging in vivo using bioluminescence, allowing for the detection of distinct events in deep tissues within the same organism.
Authors: Melanie S Evans; Joanna P Chaurette; Spencer T Adams; Gadarla R Reddy; Miranda A Paley; Neil Aronin; Jennifer A Prescher; Stephen C Miller Journal: Nat Methods Date: 2014-02-09 Impact factor: 28.547
Authors: Yichi Su; Joel R Walker; Yunhee Park; Thomas P Smith; Lan Xiang Liu; Mary P Hall; Louai Labanieh; Robin Hurst; David C Wang; Lance P Encell; Namdoo Kim; Feijie Zhang; Mark A Kay; Kerriann M Casey; Robbie G Majzner; Jennifer R Cochran; Crystal L Mackall; Thomas A Kirkland; Michael Z Lin Journal: Nat Methods Date: 2020-07-13 Impact factor: 28.547