Ann-Marie Looney1, Brian H Walsh1, Gerard Moloney2, Sue Grenham2, Ailis Fagan3, Gerard W O'Keeffe4, Gerard Clarke5, John F Cryan6, Ted G Dinan5, Geraldine B Boylan1, Deirdre M Murray7. 1. Neonatal Brain Research Group, Irish Center for Fetal and Neonatal Translational Research, Department of Pediatrics and Child Health, Cork University Maternity Hospital, Wilton, Cork, Ireland. 2. Department of Anatomy and Neuroscience, Western Gateway Building, University College Cork, Cork, Ireland. 3. Alimentary Pharmabiotic Center, University College Cork, Cork, Ireland. 4. Neonatal Brain Research Group, Irish Center for Fetal and Neonatal Translational Research, Department of Pediatrics and Child Health, Cork University Maternity Hospital, Wilton, Cork, Ireland; Department of Anatomy and Neuroscience, Western Gateway Building, University College Cork, Cork, Ireland. 5. Alimentary Pharmabiotic Center, University College Cork, Cork, Ireland; Department of Psychiatry, Biosciences Institute, University College Cork, Cork, Ireland. 6. Department of Anatomy and Neuroscience, Western Gateway Building, University College Cork, Cork, Ireland; Alimentary Pharmabiotic Center, University College Cork, Cork, Ireland. 7. Neonatal Brain Research Group, Irish Center for Fetal and Neonatal Translational Research, Department of Pediatrics and Child Health, Cork University Maternity Hospital, Wilton, Cork, Ireland. Electronic address: d.murray@ucc.ie.
Abstract
OBJECTIVE: To investigate the expression profile of microRNA (miRNA) in umbilical cord blood from infants with hypoxic ischemic encephalopathy (HIE). STUDY DESIGN: Full-term infants with perinatal asphyxia were identified under strict enrollment criteria. Degree of encephalopathy was defined using both continuous multichannel electroencephalogram in the first 24 hours of life and modified Sarnat score. Seventy infants (18 controls, 33 with perinatal asphyxia without HIE, and 19 infants with HIE [further graded as 13 mild, 2 moderate, and 4 severe]) were included in the study. MiRNA expression profiles were determined using a microarray assay and confirmed using quantitative real-time polymerase chain reaction. RESULTS: Seventy miRNAs were differentially expressed between case and control groups. Of these hsa-miR-374a was the most significantly downregulated in infants with HIE vs controls. Validation of hsa-miR-374a expression using quantitative real-time polymerase chain reaction confirmed a significant reduction in expression among infants with HIE compared with those with perinatal asphyxia and healthy controls (mean relative quantification [SD] = 0.52 [0.37] vs 1.10 [1.52] vs 1.76 [1.69], P < .02). CONCLUSIONS: We have shown a significant step-wise downregulation of hsa-miR-374a expression in cord blood of infants with perinatal asphyxia and subsequent HIE.
OBJECTIVE: To investigate the expression profile of microRNA (miRNA) in umbilical cord blood from infants with hypoxic ischemicencephalopathy (HIE). STUDY DESIGN: Full-term infants with perinatal asphyxia were identified under strict enrollment criteria. Degree of encephalopathy was defined using both continuous multichannel electroencephalogram in the first 24 hours of life and modified Sarnat score. Seventy infants (18 controls, 33 with perinatal asphyxia without HIE, and 19 infants with HIE [further graded as 13 mild, 2 moderate, and 4 severe]) were included in the study. MiRNA expression profiles were determined using a microarray assay and confirmed using quantitative real-time polymerase chain reaction. RESULTS: Seventy miRNAs were differentially expressed between case and control groups. Of these hsa-miR-374a was the most significantly downregulated in infants with HIE vs controls. Validation of hsa-miR-374a expression using quantitative real-time polymerase chain reaction confirmed a significant reduction in expression among infants with HIE compared with those with perinatal asphyxia and healthy controls (mean relative quantification [SD] = 0.52 [0.37] vs 1.10 [1.52] vs 1.76 [1.69], P < .02). CONCLUSIONS: We have shown a significant step-wise downregulation of hsa-miR-374a expression in cord blood of infants with perinatal asphyxia and subsequent HIE.
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