Nicolás Refojo1, Patricia Minervini2, Alejandra I Hevia3, Ruben A Abrantes3, Julián Fernández3, Norma Apestey2, Mariana Garnero2, Margarita Villada2, Graciela Davel3. 1. Departamento de Micología, Instituto Nacional de Enfermedades Infecciosas (INEI) Dr. Carlos G. Malbrán, Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Ciudad Autónoma de Buenos Aires, Argentina. Electronic address: nrefojo@anlis.gov.ar. 2. Hospital Oftalmológico Santa Lucía, Ciudad Autónoma de Buenos Aires, Argentina. 3. Departamento de Micología, Instituto Nacional de Enfermedades Infecciosas (INEI) Dr. Carlos G. Malbrán, Administración Nacional de Laboratorios e Institutos de Salud (ANLIS), Ciudad Autónoma de Buenos Aires, Argentina.
Abstract
BACKGROUND: Mycotic keratitis by moulds (MKM) is an important cause for corneal blindness and usually carries an unfavorable prognosis. AIMS: This study describes the risk factors and demographic and microbiological features of all MKM cases in Santa Lucía Ophthalmology Hospital during a period of 6 years. METHODS: A prospective study was performed for all MKM cases diagnosed between October 2007 and September 2013. RESULTS: Among 157 diagnosed cases, direct microscopic examination and culture were positive in 97 and 96% of the cases respectively. MKM represents 17% of all microbiologically confirmed corneal abscesses. No significant differences were detected in annual MKM frequencies across the study period, suggesting that MKM incidence remains constant over time. A male-to-female ratio was observed (2.8:1); the most affected age groups ranged from 31 to 40 years old (males) and 61-70 years old (females). The most frequent predisposing factor was trauma (40%) followed by the use of contact lenses (9%), herpetic abscesses (5%) and diabetes (4%). The predominant genera were Fusarium (66%), Aspergillus (10%), Curvularia (6%) and Alternaria (4%). The most frequent agent was Fusarium solani species complex (52%). More than two-thirds of the cases were produced by only 3 species or complexes. However, at least 29 different species were detected in the remaining cases. This is the first report of Pholiota sp. as causative agent of human MKM. CONCLUSIONS: Argentina lacks extensive epidemiological and clinical data on MKM. This six-year study performed in Argentina is a first step leading to a better understanding of MKM epidemiology in our country.
BACKGROUND:Mycotic keratitis by moulds (MKM) is an important cause for corneal blindness and usually carries an unfavorable prognosis. AIMS: This study describes the risk factors and demographic and microbiological features of all MKM cases in Santa Lucía Ophthalmology Hospital during a period of 6 years. METHODS: A prospective study was performed for all MKM cases diagnosed between October 2007 and September 2013. RESULTS: Among 157 diagnosed cases, direct microscopic examination and culture were positive in 97 and 96% of the cases respectively. MKM represents 17% of all microbiologically confirmed corneal abscesses. No significant differences were detected in annual MKM frequencies across the study period, suggesting that MKM incidence remains constant over time. A male-to-female ratio was observed (2.8:1); the most affected age groups ranged from 31 to 40 years old (males) and 61-70 years old (females). The most frequent predisposing factor was trauma (40%) followed by the use of contact lenses (9%), herpetic abscesses (5%) and diabetes (4%). The predominant genera were Fusarium (66%), Aspergillus (10%), Curvularia (6%) and Alternaria (4%). The most frequent agent was Fusarium solani species complex (52%). More than two-thirds of the cases were produced by only 3 species or complexes. However, at least 29 different species were detected in the remaining cases. This is the first report of Pholiota sp. as causative agent of human MKM. CONCLUSIONS: Argentina lacks extensive epidemiological and clinical data on MKM. This six-year study performed in Argentina is a first step leading to a better understanding of MKM epidemiology in our country.