Literature DB >> 25998947

A fluorescent tool set for yeast Atg proteins.

Dan Li1, Jing-Zhen Song, Mei-Hua Shan, Shi-Ping Li, Wei Liu, Hui Li, Jing Zhu, Yue Wang, Jianping Lin, Zhiping Xie.   

Abstract

Fluorescence microscopy of live cells is instrumental in deciphering the molecular details of autophagy. To facilitate the routine examination of yeast Atg proteins under diverse conditions, here we provide a comprehensive tool set, including (1) plasmids for the expression of GFP chimeras at endogenous levels for most Atg proteins, (2) RFP-Atg8 constructs with improved properties as a PAS marker, and (3) plasmids for the complementation of common yeast auxotrophic markers. We hope that the availability of this tool set will further accelerate yeast autophagy research.

Entities:  

Keywords:  Atg, autophagy related; C,G,R,YFP, cyan, green, red and yellow fluorescent protein; Cvt, cytoplasm-to-vacuole targeting; DsRed eExpress 2; PAS, phagophore assembly site; Vps, vacuolar protein sorting.; autophagy; auxotroph; fluorescent protein; mKO, monomeric Kusabira Orange; pseudo-monomer; starter kit; yeast

Mesh：

Substances：

Year:  2015        PMID： 25998947      PMCID： PMC4502664          DOI： 10.1080/15548627.2015.1040971

Source DB:  PubMed          Journal:  Autophagy        ISSN： 1554-8627            Impact factor:   16.016


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