Literature DB >> 25997119

Fast size-determination of intact bacterial plasmids using nanofluidic channels.

K Frykholm1, L K Nyberg, E Lagerstedt, C Noble, J Fritzsche, N Karami, T Ambjörnsson, L Sandegren, F Westerlund.   

Abstract

We demonstrate how nanofluidic channels can be used as a tool to rapidly determine the number and sizes of plasmids in bacterial isolates. Each step can be automated at low cost, opening up opportunities for general use in microbiology labs.

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Year:  2015        PMID： 25997119     DOI： 10.1039/c5lc00378d

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  6 in total

1.  A simple cut and stretch assay to detect antimicrobial resistance genes on bacterial plasmids by single-molecule fluorescence microscopy.

Authors:  Gaurav Goyal; Elina Ekedahl; My Nyblom; Jens Krog; Erik Fröbrant; Magnus Brander; Tsegaye Sewunet; Teerawit Tangkoskul; Christian G Giske; Linus Sandegren; Visanu Thamlikitkul; Tobias Ambjörnsson; Fredrik Westerlund
Journal:  Sci Rep       Date:  2022-06-03       Impact factor: 4.996

2.  Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping.

Authors:  Vilhelm Müller; Fredrika Rajer; Karolin Frykholm; Lena K Nyberg; Saair Quaderi; Joachim Fritzsche; Erik Kristiansson; Tobias Ambjörnsson; Linus Sandegren; Fredrik Westerlund
Journal:  Sci Rep       Date:  2016-12-01       Impact factor: 4.379

3.  Optical DNA Mapping Combined with Cas9-Targeted Resistance Gene Identification for Rapid Tracking of Resistance Plasmids in a Neonatal Intensive Care Unit Outbreak.

Authors:  Santosh K Bikkarolla; Viveka Nordberg; Fredrika Rajer; Vilhelm Müller; Muhammad Humaun Kabir; Sriram Kk; Albertas Dvirnas; Tobias Ambjörnsson; Christian G Giske; Lars Navér; Linus Sandegren; Fredrik Westerlund
Journal:  mBio       Date:  2019-07-09       Impact factor: 7.867

4.  Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules.

Authors:  Lena K Nyberg; Saair Quaderi; Gustav Emilsson; Nahid Karami; Erik Lagerstedt; Vilhelm Müller; Charleston Noble; Susanna Hammarberg; Adam N Nilsson; Fei Sjöberg; Joachim Fritzsche; Erik Kristiansson; Linus Sandegren; Tobias Ambjörnsson; Fredrik Westerlund
Journal:  Sci Rep       Date:  2016-07-27       Impact factor: 4.379

5.  DNA compaction by the bacteriophage protein Cox studied on the single DNA molecule level using nanofluidic channels.

Authors:  Karolin Frykholm; Ronnie Per-Arne Berntsson; Magnus Claesson; Laura de Battice; Richard Odegrip; Pål Stenmark; Fredrik Westerlund
Journal:  Nucleic Acids Res       Date:  2016-04-29       Impact factor: 16.971

6.  Optical maps of plasmids as a proxy for clonal spread of MDR bacteria: a case study of an outbreak in a rural Ethiopian hospital.

Authors:  Yii-Lih Lin; Tsegaye Sewunet; Sriram Kk; Christian G Giske; Fredrik Westerlund
Journal:  J Antimicrob Chemother       Date:  2020-10-01       Impact factor: 5.790
  6 in total

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